PSMC2 Protein (AA 2-433) (His tag)

N° du produit ABIN3086584

Détail du produit

Antigène: PSMC2 (Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 2 (PSMC2))

Type de proteíne: Recombinant

Attributs du protein: AA 2-433

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette PSMC2 protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: PDYLGADQRK TKEDEKDDKP IRALDEGDIA LLKTYGQSTY SRQIKQVEDD IQQLLKKINE LTGIKESDTG LAPPALWDLA ADKQTLQSEQ PLQVARCTKI INADSEDPKY IINVKQFAKF VVDLSDQVAP TDIEEGMRVG VDRNKYQIHI PLPPKIDPTV TMMQVEEKPD VTYSDVGGCK EQIEKLREVV ETPLLHPERF VNLGIEPPKG VLLFGPPGTG KTLCARAVAN RTDACFIRVI GSELVQKYVG EGARMVRELF EMARTKKACL IFFDEIDAIG GARFDDGAGG DNEVQRTMLE LINQLDGFDP RGNIKVLMAT NRPDTLDPAL MRPGRLDRKI EFSLPDLEGR THIFKIHARS MSVERDIRFE LLARLCPNST GAEIRSVCTE AGMFAIRARR KIATEKDFLE AVNKVIKSYA KFSATPRYMT YN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PSMC2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PSMC2 (Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 2 (PSMC2))

Autre désignation: PSMC2 (PSMC2 Produits)

Synonymes: xMSS1 Protein, psmc2 Protein, DDBDRAFT_0168337 Protein, DDBDRAFT_0232966 Protein, DDB_0168337 Protein, DDB_0232966 Protein, MSS1 Protein, Nbla10058 Protein, S7 Protein, NCU02840.1 Protein, zgc:63995 Protein, proteasome 26S subunit, ATPase 2 L homeolog Protein, proteasome (prosome, macropain) 26S subunit, ATPase 2 Protein, 26S protease regulatory subunit 7 Protein, proteasome 26S subunit, ATPase 2 Protein, regulatory particle, ATPase-like-1 Protein, psmc2.L Protein, psmc2 Protein, psmC2 Protein, PSMC2 Protein, rpt-1 Protein, GL50803_21331 Protein, EHI_080890 Protein, UREG_01412 Protein, PITG_10169 Protein, Psmc2 Protein, GL50803_86683 Protein, Tsp_09237 Protein

Sujet: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. In case of HIV-1 infection, positive modulator of Tat-mediated transactivation. {ECO:0000269|PubMed:9295362}.

Poids moléculaire: 49.5 kDa Including tag.

UniProt: P35998

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway