Proline Rich, Lacrimal 1 (PROL1) (AA 22-248) protein (His tag)

Détails pour le produit réf. ABIN3086623
Nom de la protéine
  • BPLP
  • PRL1
  • opiorphin
  • Muc10
  • opiorphin prepropeptide
  • proline rich, lacrimal 1
  • OPRPN
  • Prol1
Attributs du protein
AA 22-248
1
Origine
Humain
2
1
1
Source
Cellules d'insectes
3
1
Type de proteíne
Recombinant
Purification/Conjugué
Cette PROL1 protéine est marqué à la His tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
Séquence MQRFSRRPYL PGQLPPPPLY RPRWVPPSPP PPYDSRLNSP LSLPFVPGRV PPSSFSRFSQ AVILSQLFPL ESIRQPRLFP GYPNLHFPLR PYYVGPIRIL KPPFPPIPFF LAIYLPISNP EPQINITTAD TTITTNPPTT ATATTSTSTK PTMTISSSTV PISSTPEPAT SISAATPAAS TENTTQILAN RPHTVLLNAT VQVTTSNQTI LSSPAFKSFW QKLFAIFG HHHHHH
Sequence including C-terminal His-tag.
Attributs du produit
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human PROL1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
  • Tag Location: C-terminal His Tag
Purification The protein is purified from the cleared cell lysate using EDTA resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Pureté >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Stérilité 0.22 μm filtered
niveau d'endotoxine Protein is endotoxin-free.
Classe de qualité Crystallography grade
Nom de la protéine
Sujet Opiorphin is an endogenous inhibitor of neprilysin and aminopeptidase N. Inhibits the breakdown of substance P, Mca-BK2 and Met-enkephalin by neprilysin in vitro with IC(50) values of 29 uM, 33 uM and 33 uM respectively. Inhibits the breakdown of Ala-pNA by aminopeptidase N in vitro with an IC(50) of 65 uM. Has a potent analgesic effect when administered to rats by intravenous injection. {ECO:0000269|PubMed:17101991}.
Poids moléculaire 24.9 kD. Including tag.
UniProt Q99935
Indications d'application In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Commentaires

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles.
Stock -80 °C
Stockage commentaire Store at -80°C.
Date de péremption Unlimited (if stored properly)
Images (Fournisseur)
 image for Proline Rich, Lacrimal 1 (PROL1) (AA 22-248) protein (His tag) (ABIN3086623) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
Size-exclusion chromatography-High Pressure Liquid Chromatography (SEC-HPLC) image for Proline Rich, Lacrimal 1 (PROL1) (AA 22-248) protein (His tag) (ABIN3086623) Proline Rich, Lacrimal 1 (PROL1) (AA 22-248) protein (His tag) (Image 2)
Western Blotting (WB) image for Proline Rich, Lacrimal 1 (PROL1) (AA 22-248) protein (His tag) (ABIN3086623) Proline Rich, Lacrimal 1 (PROL1) (AA 22-248) protein (His tag) (Image 3)
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