PROL1 Protein (AA 22-248) (His tag)

N° du produit ABIN3086623

Détail du produit

Antigène: PROL1 (Proline Rich, Lacrimal 1 (PROL1))

Type de proteíne: Recombinant

Attributs du protein: AA 22-248

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette PROL1 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: MQRFSRRPYL PGQLPPPPLY RPRWVPPSPP PPYDSRLNSP LSLPFVPGRV PPSSFSRFSQ AVILSQLFPL ESIRQPRLFP GYPNLHFPLR PYYVGPIRIL KPPFPPIPFF LAIYLPISNP EPQINITTAD TTITTNPPTT ATATTSTSTK PTMTISSSTV PISSTPEPAT SISAATPAAS TENTTQILAN RPHTVLLNAT VQVTTSNQTI LSSPAFKSFW QKLFAIFG HHHHHH
Sequence including C-terminal His-tag.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PROL1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).
• Tag Location: C-terminal His Tag

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: The protein is purified from the cleared cell lysate using EDTA resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PROL1 (Proline Rich, Lacrimal 1 (PROL1))

Autre désignation: PROL1 (PROL1 Produits)

Sujet: Opiorphin is an endogenous inhibitor of neprilysin and aminopeptidase N. Inhibits the breakdown of substance P, Mca-BK2 and Met-enkephalin by neprilysin in vitro with IC(50) values of 29 uM, 33 uM and 33 uM respectively. Inhibits the breakdown of Ala-pNA by aminopeptidase N in vitro with an IC(50) of 65 uM. Has a potent analgesic effect when administered to rats by intravenous injection. {ECO:0000269|PubMed:17101991}.

Poids moléculaire: 24.9 kD. Including tag.

UniProt: Q99935