PSMA1 Protein (AA 1-263) (His tag)

N° du produit ABIN3086637

Détail du produit

Antigène: PSMA1 (Proteasome Subunit alpha Type 1 (PSMA1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-263

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette PSMA1 protéine est marqué à la His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MFRNQYDNDV TVWSPQGRIH QIEYAMEAVK QGSATVGLKS KTHAVLVALK RAQSELAAHQ KKILHVDNHI GISIAGLTAD ARLLCNFMRQ ECLDSRFVFD RPLPVSRLVS LIGSKTQIPT QRYGRRPYGV GLLIAGYDDM GPHIFQTCPS ANYFDCRAMS IGARSQSART YLERHMSEFM ECNLNELVKH GLRALRETLP AEQDLTTKNV SIGIVGKDLE FTIYDDDDVS PFLEGLEERP QRKAQPAQPA DEPAEKADEP MEH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PSMA1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PSMA1 (Proteasome Subunit alpha Type 1 (PSMA1))

Autre désignation: PSMA1 (PSMA1 Produits)

Synonymes: PSMA1 Protein, pros30 Protein, DDBDRAFT_0204226 Protein, DDBDRAFT_0214956 Protein, DDB_0204226 Protein, DDB_0214956 Protein, C2 Protein, HC2 Protein, Pros-30 Protein, alpha-type Protein, NU Protein, PROS30 Protein, CC2 Protein, zgc:92726 Protein, 143314_at Protein, Alpha-6 Protein, CG4904 Protein, Dmel\CG4904 Protein, PROS-35 Protein, PROS-Dm35 Protein, Pros-35 Protein, Pros-Dm35 Protein, Prosalpha6 Protein, alpha6 Protein, alpha_dm Protein, anon-SAGE:Wang-116 Protein, pros 35 Protein, pros35 Protein, proteasome subunit alpha 1 Protein, proteasome subunit alpha type 1 Protein, 20S proteasome subunit C2 Protein, proteasome (prosome, macropain) subunit, alpha type 1 Protein, proteasome subunit alpha 1 L homeolog Protein, Proteasome alpha6 subunit Protein, PSMA1 Protein, psma1 Protein, CNB05540 Protein, psmA1 Protein, Psma1 Protein, psma1.L Protein, Prosalpha6 Protein

Sujet: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH . The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells (By similarity). {ECO:0000250}.

Poids moléculaire: 30.5 kDa Including tag.

UniProt: P25786

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA