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PTPRN2 Protein (AA 637-1015) (His tag)

Crystallography grade PTPRN2 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
N° du produit ABIN3086812
  • Antigène Voir toutes PTPRN2 Protéines
    PTPRN2 (Protein tyrosine Phosphatase, Receptor Type, N Polypeptide 2 (PTPRN2))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 637-1015
    Origine
    • 6
    • 5
    • 2
    Humain
    Source
    • 4
    • 4
    • 3
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette PTPRN2 protéine est marqué à la His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Séquence
    YCLRHSSQHR LKEKLSGLGG DPGADATAAY QELCRQRMAT RPPDRPEGPH TSRISSVSSQ FSDGPIPSPS ARSSASSWSE EPVQSNMDIS TGHMILSYME DHLKNKNRLE KEWEALCAYQ AEPNSSFVAQ REENVPKNRS LAVLTYDHSR VLLKAENSHS HSDYINASPI MDHDPRNPAY IATQGPLPAT VADFWQMVWE SGCVVIVMLT PLAENGVRQC YHYWPDEGSN LYHIYEVNLV SEHIWCEDFL VRSFYLKNLQ TNETRTVTQF HFLSWYDRGV PSSSRSLLDF RRKVNKCYRG RSCPIIVHCS DGAGRSGTYV LIDMVLNKMA KGAKEIDIAA TLEHLRDQRP GMVQTKEQFE FALTAVAEEV NAILKALPQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PTPRN2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product PTPRN2 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    PTPRN2 (Protein tyrosine Phosphatase, Receptor Type, N Polypeptide 2 (PTPRN2))
    Autre désignation
    PTPRN2 (PTPRN2 Produits)
    Synonymes
    IA-2beta Protein, IAR Protein, ICAAR Protein, PTPRP Protein, R-PTP-N2 Protein, 4930425H11Rik Protein, IA2beta Protein, PTP-NP Protein, Phol Protein, mKIAA0387 Protein, phogrin Protein, protein tyrosine phosphatase, receptor type N2 Protein, protein tyrosine phosphatase, receptor type, N polypeptide 2 Protein, PTPRN2 Protein, Ptprn2 Protein
    Sujet
    Plays a role in vesicle-mediated secretory processes. Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets. Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation. Plays a role in insulin secretion in response to glucose stimuli. Required for normal accumulation of the neurotransmitters norepinephrine, dopamine and serotonin in the brain. In females, but not in males, required for normal accumulation and secretion of pituitary hormones, such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH). {ECO:0000250|UniProtKB:P80560}.
    Poids moléculaire
    43.8 kDa Including tag.
    UniProt
    Q92932
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