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Serotonin Receptor 1A Protein (HTR1A) (AA 1-422) (rho-1D4 tag)

Crystallography grade HTR1A Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N° du produit ABIN3087934
  • Antigène Voir toutes Serotonin Receptor 1A (HTR1A) Protéines
    Serotonin Receptor 1A (HTR1A)
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-422
    Origine
    • 7
    • 3
    • 1
    Humain
    Source
    • 4
    • 3
    • 2
    Cellules d'insectes
    Purification/Conjugué
    Cette Serotonin Receptor 1A protéine est marqué à la rho-1D4 tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Séquence
    MDVLSPGQGN NTTSPPAPFE TGGNTTGISD VTVSYQVITS LLLGTLIFCA VLGNACVVAA IALERSLQNV ANYLIGSLAV TDLMVSVLVL PMAALYQVLN KWTLGQVTCD LFIALDVLCC TSSILHLCAI ALDRYWAITD PIDYVNKRTP RRAAALISLT WLIGFLISIP PMLGWRTPED RSDPDACTIS KDHGYTIYST FGAFYIPLLL MLVLYGRIFR AARFRIRKTV KKVEKTGADT RHGASPAPQP KKSVNGESGS RNWRLGVESK AGGALCANGA VRQGDDGAAL EVIEVHRVGN SKEHLPLPSE AGPTPCAPAS FERKNERNAE AKRKMALARE RKTVKTLGII MGTFILCWLP FFIVALVLPF CESSCHMPTL LGAIINWLGY SNSLLNPVIY AYFNKDFQNA FKKIIKCKFC RQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human HTR1A Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin-free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product HTR1A Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    Serotonin Receptor 1A (HTR1A)
    Autre désignation
    HTR1A (HTR1A Produits)
    Synonymes
    5-HT-dro2A Protein, 5-HT1ADro Protein, 5-HT[1A]Dro Protein, 5-HT[[1ADro]] Protein, 5-HT[[1A]] Protein, 5-HT[[1A]]DRO Protein, 5HT-R2A Protein, 5HT-dro2A Protein, 5HT-drp[[2A]] Protein, 5HT1A Protein, 5HT[[1ADro]] Protein, 5htdro2a Protein, CG16720 Protein, DRO2A Protein, Dm5HTdro2A Protein, Dmel\\CG16720 Protein, d5-HT1A Protein, 5htr1a Protein, htr1a Protein, HTR1A Protein, Htr1a Protein, 5-Ht1a Protein, Gpcr18 Protein, 5-HT-1A Protein, 5-HT1A Protein, 5HT1a Protein, ADRB2RL1 Protein, ADRBRL1 Protein, G-21 Protein, PFMCD Protein, RAT5HT1A Protein, 5ht1a Protein, adrb2rl1 Protein, adrbrl1 Protein, htr1a-A Protein, 5-hydroxytryptamine (serotonin) receptor 1A Protein, 5-hydroxytryptamine receptor 1A Protein, 5-hydroxytryptamine (serotonin) receptor 1A a Protein, 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled L homeolog Protein, 5-HT1A Protein, HTR1A Protein, htr1aa Protein, Htr1a Protein, htr1a.L Protein
    Sujet
    G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli. {ECO:0000269|PubMed:22957663, ECO:0000269|PubMed:3138543, ECO:0000269|PubMed:8138923, ECO:0000269|PubMed:8393041}.
    Poids moléculaire
    47.3 kDa Including tag.
    UniProt
    P08908
    Pathways
    Signalistation JAK/STAT, Synaptic Membrane, Feeding Behaviour
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