ACSS1 Protein (AA 38-689) (His tag)

N° du produit ABIN3088341

Détail du produit

Antigène: ACSS1 (Acyl-CoA Synthetase Short-Chain Family Member 1 (ACSS1))

Type de proteíne: Recombinant

Attributs du protein: AA 38-689

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette ACSS1 protéine est marqué à la His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: ASGPSGSAPA VAAAAAQPGS YPALSAQAAR EPAAFWGPLA RDTLVWDTPY HTVWDCDFST GKIGWFLGGQ LNVSVNCLDQ HVRKSPESVA LIWERDEPGT EVRITYRELL ETTCRLANTL KRHGVHRGDR VAIYMPVSPL AVAAMLACAR IGAVHTVIFA GFSAESLAGR INDAKCKVVI TFNQGLRGGR VVELKKIVDE AVKHCPTVQH VLVAHRTDNK VHMGDLDVPL EQEMAKEDPV CAPESMGSED MLFMLYTSGS TGMPKGIVHT QAGYLLYAAL THKLVFDHQP GDIFGCVADI GWITGHSYVV YGPLCNGATS VLFESTPVYP NAGRYWETVE RLKINQFYGA PTAVRLLLKY GDAWVKKYDR SSLRTLGSVG EPINCEAWEW LHRVVGDSRC TLVDTWWQTE TGGICIAPRP SEEGAEILPA MAMRPFFGIV PVLMDEKGSV VEGSNVSGAL CISQAWPGMA RTIYGDHQRF VDAYFKAYPG YYFTGDGAYR TEGGYYQITG RMDDVINISG HRLGTAEIED AIADHPAVPE SAVIGYPHDI KGEAAFAFIV VKDSAGDSDV VVQELKSMVA TKIAKYAVPD EILVVKRLPK TRSGKVMRRL LRKIITSEAQ ELGDTTTLED PSIIAEILSV YQKCKDKQAA AK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ACSS1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ACSS1 (Acyl-CoA Synthetase Short-Chain Family Member 1 (ACSS1))

Autre désignation: ACSS1 (ACSS1 Produits)

Synonymes: ACAS2L Protein, cb395 Protein, sb:cb395 Protein, wu:fa05a10 Protein, wu:fc49f03 Protein, wu:fi28d10 Protein, zgc:113194 Protein, zgc:158738 Protein, ACECS1 Protein, AceCS2L Protein, 1110032O15Rik Protein, AI788978 Protein, Acas2 Protein, Acas2l Protein, AceCS2 Protein, acyl-CoA synthetase short chain family member 1 Protein, acyl-CoA synthetase short-chain family member 1 Protein, acetyl-coenzyme A synthetase 2-like, mitochondrial Protein, ACSS1 Protein, acss1 Protein, LOC100541607 Protein, Acss1 Protein

Sujet: Important for maintaining normal body temperature during fasting and for energy homeostasis. Essential for energy expenditure under ketogenic conditions (By similarity). Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO(2). {ECO:0000250, ECO:0000269|PubMed:16788062}.

Poids moléculaire: 72.1 kDa Including tag.

UniProt: Q9NUB1