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LPHN2 Protein (AA 26-1459) (rho-1D4 tag)

Crystallography grade LPHN2 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
N° du produit ABIN3088446
  • Antigène Voir toutes LPHN2 Protéines
    LPHN2 (Latrophilin 2 (LPHN2))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 26-1459
    Origine
    • 3
    • 2
    • 1
    Humain
    Source
    • 5
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette LPHN2 protéine est marqué à la rho-1D4 tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Séquence
    FSRAALPFGL VRRELSCEGY SIDLRCPGSD VIMIESANYG RTDDKICDAD PFQMENTDCY LPDAFKIMTQ RCNNRTQCIV VTGSDVFPDP CPGTYKYLEV QYECVPYIFV CPGTLKAIVD SPCIYEAEQK AGAWCKDPLQ AADKIYFMPW TPYRTDTLIE YASLEDFQNS RQTTTYKLPN RVDGTGFVVY DGAVFFNKER TRNIVKFDLR TRIKSGEAII NYANYHDTSP YRWGGKTDID LAVDENGLWV IYATEQNNGM IVISQLNPYT LRFEATWETV YDKRAASNAF MICGVLYVVR SVYQDNESET GKNSIDYIYN TRLNRGEYVD VPFPNQYQYI AAVDYNPRDN QLYVWNNNFI LRYSLEFGPP DPAQVPTTAV TITSSAELFK TIISTTSTTS QKGPMSTTVA GSQEGSKGTK PPPAVSTTKI PPITNIFPLP ERFCEALDSK GIKWPQTQRG MMVERPCPKG TRGTASYLCM ISTGTWNPKG PDLSNCTSHW VNQLAQKIRS GENAASLANE LAKHTKGPVF AGDVSSSVRL MEQLVDILDA QLQELKPSEK DSAGRSYNKL QKREKTCRAY LKAIVDTVDN LLRPEALESW KHMNSSEQAH TATMLLDTLE EGAFVLADNL LEPTRVSMPT ENIVLEVAVL STEGQIQDFK FPLGIKGAGS SIQLSANTVK QNSRNGLAKL VFIIYRSLGQ FLSTENATIK LGADFIGRNS TIAVNSHVIS VSINKESSRV YLTDPVLFTL PHIDPDNYFN ANCSFWNYSE RTMMGYWSTQ GCKLVDTNKT RTTCACSHLT NFAILMAHRE IAYKDGVHEL LLTVITWVGI VISLVCLAIC IFTFCFFRGL QSDRNTIHKN LCINLFIAEF IFLIGIDKTK YAIACPIFAG LLHFFFLAAF AWMCLEGVQL YLMLVEVFES EYSRKKYYYV AGYLFPATVV GVSAAIDYKS YGTEKACWLH VDNYFIWSFI GPVTFIILLN IIFLVITLCK MVKHSNTLKP DSSRLENIKS WVLGAFALLC LLGLTWSFGL LFINEETIVM AYLFTIFNAF QGVFIFIFHC ALQKKVRKEY GKCFRHSYCC GGLPTESPHS SVKASTTRTS ARYSSGTQSR IRRMWNDTVR KQSESSFISG DINSTSTLNQ GMTGNYLLTN PLLRPHGTNN PYNTLLAETV VCNAPSAPVF NSPGHSLNNA RDTSAMDTLP LNGNFNNSYS LHKGDYNDSV QVVDCGLSLN DTAFEKMIIS ELVHNNLRGS SKTHNLELTL PVKPVIGGSS SEDDAIVADA SSLMHSDNPG LELHHKELEA PLIPQRTHSL LYQPQKKVKS EGTDSYVSQL TAEAEDHLQS PNRDSLYTSM PNLRDSPYPE SSPDMEEDLS PSRRSENEDI YYKSMPNLGA GHQLQMCYQI SRGNSDGYII PINKEGCIPE GDVREGQMQL VTSL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ADGRL2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin-free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product LPHN2 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    LPHN2 (Latrophilin 2 (LPHN2))
    Autre désignation
    ADGRL2 (LPHN2 Produits)
    Synonymes
    LPHN2 Protein, CIRL2 Protein, CL2 Protein, LEC1 Protein, LPHH1 Protein, AI450192 Protein, Gm619 Protein, Lec1 Protein, Lphh1 Protein, mKIAA0786 Protein, Cirl-2 Protein, Cirl2 Protein, Cl2ac Protein, LEC2 Protein, adhesion G protein-coupled receptor L2 Protein, ADGRL2 Protein, adgrl2 Protein, LOC100538279 Protein, Adgrl2 Protein
    Sujet
    Calcium-independent receptor of low affinity for alpha-latrotoxin, an excitatory neurotoxin present in black widow spider venom which triggers massive exocytosis from neurons and neuroendocrine cells. Receptor propably implicated in the regulation of exocytosis. {ECO:0000250|UniProtKB:O88923}.
    Poids moléculaire
    161.7 kDa Including tag.
    UniProt
    O95490
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