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ADRA1B Protein (AA 352-520) (His tag)

Crystallography grade ADRA1B Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
N° du produit ABIN3088675
  • Antigène Voir toutes ADRA1B Protéines
    ADRA1B (Adrenergic, alpha-1B-, Receptor (ADRA1B))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 352-520
    Origine
    • 2
    • 1
    Humain
    Source
    • 3
    Cellules d'insectes
    Purification/Conjugué
    Cette ADRA1B protéine est marqué à la His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Séquence
    SKEFKRAFVR ILGCQCRGRG RRRRRRRRRL GGCAYTYRPW TRGGSLERSQ SRKDSLDDSG SCLSGSQRTL PSASPSPGYL GRGAPPPVEL CAFPEWKAPG ALLSLPAPEP PGRRGRHDSG PLFTFKLLTE PESPGTDGGA SNGGCEAAAD VANGQPGFKS NMPLAPGQF
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ADRA1B Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    ADRA1B (Adrenergic, alpha-1B-, Receptor (ADRA1B))
    Autre désignation
    ADRA1B (ADRA1B Produits)
    Synonymes
    LOC100136712 Protein, [a]1b Protein, ADRA1 Protein, ALPHA1BAR Protein, RDC5 Protein, alpha-1B adrenoceptor Protein, adrenoceptor alpha 1B Protein, adrenergic receptor, alpha 1b Protein, LOC100136712 Protein, ADRA1B Protein, Adra1b Protein
    Sujet
    This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes. {ECO:0000269|PubMed:18802028, ECO:0000269|PubMed:22120526}.
    Poids moléculaire
    19.1 kDa Including tag.
    UniProt
    P35368
    Pathways
    AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
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