ADCY10 Protein (AA 1-1610) (Strep Tag)

N° du produit ABIN3088760

Détail du produit

Antigène: ADCY10 (Adenylate Cyclase 10 (Soluble) (ADCY10))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1610

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette ADCY10 protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Séquence: MNTPKEEFQD WPIVRIAAHL PDLIVYGHFS PERPFMDYFD GVLMFVDISG FTAMTEKFSS AMYMDRGAEQ LVEILNYHIS AIVEKVLIFG GDILKFAGDA LLALWRVERK QLKNIITVVI KCSLEIHGLF ETQEWEEGLD IRVKIGLAAG HISMLVFGDE THSHFLVIGQ AVDDVRLAQN MAQMNDVILS PNCWQLCDRS MIEIESVPDQ RAVKVNFLKP PPNFNFDEFF TKCTTFMHYY PSGEHKNLLR LACTLKPDPE LEMSLQKYVM ESILKQIDNK QLQGYLSELR PVTIVFVNLM FEDQDKAEEI GPAIQDAYMH ITSVLKIFQG QINKVFMFDK GCSFLCVFGF PGEKVPDELT HALECAMDIF DFCSQVHKIQ TVSIGVASGI VFCGIVGHTV RHEYTVIGQK VNLAARMMMY YPGIVTCDSV TYNGSNLPAY FFKELPKKVM KGVADSGPLY QYWGRTEKVM FGMACLICNR KEDYPLLGRN KEINYFMYTM KKFLISNSSQ VLMYEGLPGY GKSQILMKIE YLAQGKNHRI IAISLNKISF HQTFYTIQMF MANVLGLDTC KHYKERQTNL RNKVMTLLDE KFYCLLNDIF HVQFPISREI SRMSTLKKQK QLEILFMKIL KLIVKEERII FIIDEAQFVD STSWRFMEKL IRTLPIFIIM SLCPFVNIPC AAARAVIKNR NTTYIVIGAV QPNDISNKIC LDLNVSCISK ELDSYLGEGS CGIPFYCEEL LKNLEHHEVL VFQQTESEEK TNRTWNNLFK YSIKLTEKLN MVTLHSDKES EEVCHLTSGV RLKNLSPPTS LKEISLIQLD SMRLSHQMLV RCAAIIGLTF TTELLFEILP CWNMKMMIKT LATLVESNIF YCFRNGKELQ KALKQNDPSF EVHYRSLSLK PSEGMDHGEE EQLRELENEV IECHRIRFCN PMMQKTAYEL WLKDQRKAMH LKCARFLEED AHRCDHCRGR DFIPYHHFTV NIRLNALDMD AIKKMAMSHG FKTEEKLILS NSEIPETSAF FPENRSPEEI REKILNFFDH VLTKMKTSDE DIIPLESCQC EEILEIVILP LAHHFLALGE NDKALYYFLE IASAYLIFCD NYMAYMYLNE GQKLLKTLKK DKSWSQTFES ATFYSLKGEV CFNMGQIVLA KKMLRKALKL LNRIFPYNLI SLFLHIHVEK NRHFHYVNRQ AQESPPPGKK RLAQLYRQTV CLSLLWRIYS YSYLFHCKYY AHLAVMMQMN TALETQNCFQ IIKAYLDYSL YHHLAGYKGV WFKYEVMAME HIFNLPLKGE GIEIVAYVAE TLVFNKLIMG HLDLAIELGS RALQMWALLQ NPNRHYQSLC RLSRCLLLNS RYPQLIQVLG RLWELSVTQE HIFSKAFFYF VCLDILLYSG FVYRTFEECL EFIHQYENNR ILKFHSGLLL GLYSSVAIWY ARLQEWDNFY KFSNRAKNLL PRRTMTLTYY DGISRYMEGQ VLHLQKQIKE QSENAQASGE ELLKNLENLV AQNTTGPVFC PRLYHLMAYV CILMGDGQKC GLFLNTALRL SETQGNILEK CWLNMNKESW YSTSELKEDQ WLQTILSLPS WEKIVAGRVN IQDLQKNKFL MRANTVDNHF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ADCY10 (Adenylate Cyclase 10 (Soluble) (ADCY10))

Autre désignation: ADCY10 (ADCY10 Produits)

Synonymes: HCA2 Protein, RP1-313L4.2 Protein, SAC Protein, SACI Protein, Sacy Protein, Sac Protein, 4930431D04Rik Protein, 4931412F17 Protein, sAC Protein, ADCY10 Protein, SACY Protein, adenylate cyclase 10 Protein, adenylate cyclase 10 (soluble) Protein, ADCY10 Protein, Adcy10 Protein

Sujet: Adenylate cyclase type 10 (EC 4.6.1.1) (AH-related protein) (Adenylate cyclase homolog) (Germ cell soluble adenylyl cyclase) (hsAC) (sAC) (Testicular soluble adenylyl cyclase),FUNCTION: Catalyzes the formation of the signaling molecule cAMP (PubMed:12609998, PubMed:15659711, PubMed:24616449, PubMed:25040695, PubMed:24567411). May function as sensor that mediates responses to changes in cellular bicarbonate and CO(2) levels (PubMed:15659711, PubMed:17591988). Has a critical role in mammalian spermatogenesis by producing the cAMP which regulates cAMP-responsive nuclear factors indispensable for sperm maturation in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization (By similarity). Involved in ciliary beat regulation (PubMed:17591988). {ECO:0000250|UniProtKB:Q8C0T9, ECO:0000269|PubMed:15659711, ECO:0000269|PubMed:17591988, ECO:0000269|PubMed:24567411, ECO:0000269|PubMed:24616449, ECO:0000269|PubMed:25040695}.

Poids moléculaire: 187.1 kDa

UniProt: Q96PN6

Pathways: cAMP Metabolic Process