ALT Protein (AA 2-496) (His tag)

N° du produit ABIN3088852

Détail du produit

Antigène: ALT (Alanine Aminotransferase (ALT))

Type de proteíne: Recombinant

Attributs du protein: AA 2-496

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette ALT protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: ASSTGDRSQA VRHGLRAKVL TLDGMNPRVR RVEYAVRGPI VQRALELEQE LRQGVKKPFT EVIRANIGDA QAMGQRPITF LRQVLALCVN PDLLSSPNFP DDAKKRAERI LQACGGHSLG AYSVSSGIQL IREDVARYIE RRDGGIPADP NNVFLSTGAS DAIVTVLKLL VAGEGHTRTG VLIPIPQYPL YSATLAELGA VQVDYYLDEE RAWALDVAEL HRALGQARDH CRPRALCVIN PGNPTGQVQT RECIEAVIRF AFEERLFLLA DEVYQDNVYA AGSQFHSFKK VLMEMGPPYA GQQELASFHS TSKGYMGECG FRGGYVEVVN MDAAVQQQML KLMSVRLCPP VPGQALLDLV VSPPAPTDPS FAQFQAEKQA VLAELAAKAK LTEQVFNEAP GISCNPVQGA MYSFPRVQLP PRAVERAQEL GLAPDMFFCL RLLEETGICV VPGSGFGQRE GTYHFRMTIL PPLEKLRLLL EKLSRFHAKF TLEYS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human GPT Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ALT (Alanine Aminotransferase (ALT))

Autre désignation: GPT (ALT Produits)

Synonymes: AAT1 Protein, ALT1 Protein, GPT1 Protein, GPT Protein, Gpt1 Protein, An11g02620 Protein, AO090003000164 Protein, 233.t00009 Protein, 24.t00016 Protein, 1300007J06Rik Protein, 2310022B03Rik Protein, ALT Protein, Gpt-1 Protein, AU021132 Protein, Dpagt2 Protein, Gnpta Protein, gpt Protein, ALANINE AMINOTRANSFERAS Protein, AtAlaAT1 Protein, AtAlaATc Protein, T13M22.3 Protein, T13M22_3 Protein, alanine aminotransferas Protein, glutamic--pyruvic transaminase Protein, glutamic-pyruvate transaminase (alanine aminotransferase) Protein, alanine aminotransferase Protein, alanine aminotransferase 1 Protein, glutamic pyruvic transaminase, soluble Protein, dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) Protein, alanine aminotransferase 2-like Protein, Alanine aminotransferase 1, mitochondrial Protein, GPT Protein, Gpt Protein, Tb927.1.3950 Protein, ANI_1_368094 Protein, AOR_1_284154 Protein, ALAT_1 Protein, ALAAT1 Protein, EHI_159710 Protein, EHI_096750 Protein, LOC5572541 Protein, ALAT Protein, AAT2 Protein, Dpagt1 Protein, LOC100537633 Protein, AlaAT1 Protein

Sujet: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity). {ECO:0000250}.

Poids moléculaire: 55.5 kDa Including tag.

UniProt: P24298