ARG2 Protein (AA 23-354) (His tag)

N° du produit ABIN3089150

Détail du produit

Antigène: ARG2 (Arginase, Type II (ARG2))

Type de proteíne: Recombinant

Attributs du protein: AA 23-354

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette ARG2 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: VHSVAVIGAP FSQGQKRKGV EHGPAAIREA GLMKRLSSLG CHLKDFGDLS FTPVPKDDLY NNLIVNPRSV GLANQELAEV VSRAVSDGYS CVTLGGDHSL AIGTISGHAR HCPDLCVVWV DAHADINTPL TTSSGNLHGQ PVSFLLRELQ DKVPQLPGFS WIKPCISSAS IVYIGLRDVD PPEHFILKNY DIQYFSMRDI DRLGIQKVME RTFDLLIGKR QRPIHLSFDI DAFDPTLAPA TGTPVVGGLT YREGMYIAEE IHNTGLLSAL DLVEVNPQLA TSEEEAKTTA NLAVDVIASS FGQTREGGHI VYDQLPTPSS PDESENQARV RI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ARG2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ARG2 (Arginase, Type II (ARG2))

Autre désignation: ARG2 (ARG2 Produits)

Synonymes: AII Protein, AU022422 Protein, zgc:65793 Protein, zgc:85630 Protein, wu:fb67g02 Protein, ARG2 Protein, LeARG2 Protein, arg1 Protein, arg3 Protein, arg2 Protein, arg2-c Protein, arg2-b Protein, arginase 2 Protein, arginase type II Protein, arginase 2 L homeolog Protein, arginase 2 S homeolog Protein, ARG2 Protein, Arg2 Protein, arg2 Protein, arg2.L Protein, arg2.S Protein

Sujet: May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase. Since NO synthase is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase II plays a role in both male and female sexual arousal. It is therefore a potential target for the treatment of male and female sexual arousal disorders. {ECO:0000269|PubMed:12859189}.

Poids moléculaire: 37.0 kDa Including tag.

UniProt: P78540