ACTR3 Protein (AA 2-418) (His tag)

N° du produit ABIN3089211

Détail du produit

Antigène: ACTR3 (ARP3 Actin-Related Protein 3 (ACTR3))

Type de proteíne: Recombinant

Attributs du protein: AA 2-418

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette ACTR3 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: AGRLPACVVD CGTGYTKLGY AGNTEPQFII PSCIAIKESA KVGDQAQRRV MKGVDDLDFF IGDEAIEKPT YATKWPIRHG IVEDWDLMER FMEQVIFKYL RAEPEDHYFL LTEPPLNTPE NREYTAEIMF ESFNVPGLYI AVQAVLALAA SWTSRQVGER TLTGTVIDSG DGVTHVIPVA EGYVIGSCIK HIPIAGRDIT YFIQQLLRDR EVGIPPEQSL ETAKAVKERY SYVCPDLVKE FNKYDTDGSK WIKQYTGINA ISKKEFSIDV GYERFLGPEI FFHPEFANPD FTQPISEVVD EVIQNCPIDV RRPLYKNIVL SGGSTMFRDF GRRLQRDLKR TVDARLKLSE ELSGGRLKPK PIDVQVITHH MQRYAVWFGG SMLASTPEFY QVCHTKKDYE EIGPSICRHN PVFGVMS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ACTR3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ACTR3 (ARP3 Actin-Related Protein 3 (ACTR3))

Autre désignation: ACTR3 (ACTR3 Produits)

Synonymes: ARP3 Protein, 1200003A09Rik Protein, Arp3 Protein, DDBDRAFT_0218534 Protein, DDBDRAFT_0219936 Protein, DDB_0218534 Protein, DDB_0219936 Protein, actr3 Protein, MGC53892 Protein, ACTR3 Protein, ACTIN-RELATED PROTEIN 3 Protein, ARABIDOPSIS THALIANA ACTIN-RELATED PROTEIN 3 Protein, ATARP3 Protein, DISTORTED TRICHOMES 1 Protein, F3F19.20 Protein, F3F19_20 Protein, ARP3 actin related protein 3 homolog Protein, ARP3 actin-related protein 3 Protein, actin-like protein 3 Protein, Actin-like protein 3 Protein, actin related protein 3 Protein, ARP3 actin-related protein 3 homolog S homeolog Protein, Actin-like ATPase superfamily protein Protein, ACTR3 Protein, Actr3 Protein, Tb09.160.3850 Protein, TVAG_371880 Protein, arpC Protein, LOC100281262 Protein, actr3.S Protein, actr3 Protein, DIS1 Protein

Sujet: Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis. {ECO:0000269|PubMed:20393563, ECO:0000269|PubMed:9000076}.

Poids moléculaire: 48.2 kDa Including tag.

UniProt: P61158

Pathways: Signalisation RTK, Regulation of Actin Filament Polymerization