ASIC1 Protein (AA 70-427) (His tag)

N° du produit ABIN3089318

Détail du produit

Antigène: ASIC1 (ACCN2) (Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))

Type de proteíne: Recombinant

Attributs du protein: AA 70-427

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette ASIC1 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: HYHHVTKLDE VAASQLTFPA VTLCNLNEFR FSQVSKNDLY HAGELLALLN NRYEIPDTQM ADEKQLEILQ DKANFRSFKP KPFNMREFYD RAGHDIRDML LSCHFRGEVC SAEDFKVVFT RYGKCYTFNS GRDGRPRLKT MKGGTGNGLE IMLDIQQDEY LPVWGETDET SFEAGIKVQI HSQDEPPFID QLGFGVAPGF QTFVACQEQR LIYLPPPWGT CKAVTMDSDL DFFDSYSITA CRIDCETRYL VENCNCRMVH MPGDAPYCTP EQYKECADPA LDFLVEKDQE YCVCEMPCNL TRYGKELSMV KIPSKASAKY LAKKFNKSEQ YIGENILVLD IFFEVLNYET IEQKKAYE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ASIC1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ASIC1 (ACCN2) (Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))

Autre désignation: ASIC1 (ACCN2 Produits)

Synonymes: ACCN2 Protein, ASIC Protein, BNaC2 Protein, Accn2 Protein, ASIC1 Protein, AI843610 Protein, ASIC1a Protein, B530003N02Rik Protein, acid sensing ion channel subunit 1 Protein, acid-sensing (proton-gated) ion channel 1 Protein, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 Protein, ASIC1 Protein, Asic1 Protein, asic1 Protein, SMARCD1 Protein

Sujet: Isoform 2 and isoform 3 function as proton-gated sodium channels, they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear. {ECO:0000269|PubMed:22760635}., Isoform 1 does not display proton-gated cation channel activity. {ECO:0000269|PubMed:22760635}.

Poids moléculaire: 42.2 kDa Including tag.

UniProt: P78348