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ARPC3 Protein (AA 2-178) (His tag)

Crystallography grade ARPC3 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
N° du produit ABIN3089469
  • Antigène Voir toutes ARPC3 Protéines
    ARPC3 (Actin Related Protein 2/3 Complex, Subunit 3, 21kDa (ARPC3))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-178
    Origine
    • 6
    • 1
    • 1
    Humain
    Source
    • 3
    • 3
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette ARPC3 protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Séquence
    PAYHSSLMDP DTKLIGNMAL LPIRSQFKGP APRETKDTDI VDEAIYYFKA NVFFKNYEIK NEADRTLIYI TLYISECLKK LQKCNSKSQG EKEMYTLGIT NFPIPGEPGF PLNAIYAKPA NKQEDEVMRA YLQQLRQETG LRLCEKVFDP QNDKPSKWWT CFVKRQFMNK SLSGPGQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ARPC3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product ARPC3 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    ARPC3 (Actin Related Protein 2/3 Complex, Subunit 3, 21kDa (ARPC3))
    Autre désignation
    ARPC3 (ARPC3 Produits)
    Synonymes
    ARC21 Protein, p21-Arc Protein, 1110006A04Rik Protein, 21kDa Protein, AA408672 Protein, AI788639 Protein, p21-Ar Protein, p21Arc Protein, arc21 Protein, p21-arc Protein, zgc:86828 Protein, actin related protein 2/3 complex subunit 3 Protein, actin related protein 2/3 complex, subunit 3 Protein, actin related protein 2/3 complex subunit 3 L homeolog Protein, actin related protein 2/3 complex, subunit 3, 21kDa Protein, actin related protein 2/3 complex subunit 3 pseudogene Protein, actin-related protein 2/3 complex subunit 3 Protein, actin-related protein 2/3 complex subunit 3 pseudogene Protein, ARPC3 Protein, Arpc3 Protein, arpc3.L Protein, LOC738738 Protein, arpc3 Protein, LOC100036831 Protein, LOC100341254 Protein
    Sujet
    Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
    Poids moléculaire
    21.4 kDa Including tag.
    UniProt
    O15145
    Pathways
    Signalisation RTK, Regulation of Actin Filament Polymerization
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