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ATG10 Protein (AA 1-220) (His tag)

Crystallography grade ATG10 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
N° du produit ABIN3089492
  • Antigène Voir toutes ATG10 Protéines
    ATG10 (Autophagy Related 10 (ATG10))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-220
    Origine
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Source
    • 5
    • 4
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette ATG10 protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Séquence
    MEEDEFIGEK TFQRYCAEFI KHSQQIGDSW EWRPSKDCSD GYMCKIHFQI KNGSVMSHLG ASTHGQTCLP MEEAFELPLD DCEVIETAAA SEVIKYEYHV LYSCSYQVPV LYFRASFLDG RPLTLKDIWE GVHECYKMRL LQGPWDTITQ QEHPILGQPF FVLHPCKTNE FMTPVLKNSQ KINKNVNYIT SWLSIVGPVV GLNLPLSYAK ATSQDERNVP
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ATG10 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product ATG10 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    ATG10 (Autophagy Related 10 (ATG10))
    Autre désignation
    ATG10 (ATG10 Produits)
    Synonymes
    APG10 Protein, APG10L Protein, pp12616 Protein, Atg10 Protein, ATG10 Protein, MGC123341 Protein, im:7153729 Protein, zgc:123341 Protein, ATATG10 Protein, autophagy 10 Protein, DDBDRAFT_0190076 Protein, DDBDRAFT_0252608 Protein, DDB_0190076 Protein, DDB_0252608 Protein, 5330424L23Rik Protein, 5430428K15Rik Protein, AI852123 Protein, Agp10 Protein, Apg10l Protein, Apg10p Protein, Atg10l Protein, autophagy related 10 Protein, autophagy-related 10 Protein, AuTophaGy (yeast Atg homolog) Protein, ATG10 autophagy related 10 homolog (S. cerevisiae) Protein, autophagocytosis-associated family protein Protein, E2-like conjugating enzyme Protein, hypothetical protein Protein, autophagy protein 10 Protein, autophagy-related protein 10 Protein, ATG10 Protein, Atg10 Protein, atg-10 Protein, atg10 Protein, CAALFM_C302930WA Protein, APG10 Protein
    Sujet
    E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis. {ECO:0000250, ECO:0000269|PubMed:21367888}.
    Poids moléculaire
    26.2 kDa Including tag.
    UniProt
    Q9H0Y0
    Pathways
    ER-Nucleus Signaling, Autophagy
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