beta-1,4-N-Acetyl-Galactosaminyl Transferase 4 (B4GALNT4) (AA 37-1039) protein (His tag)

N° du produit ABIN3089825

Détail du produit

Antigène: beta-1,4-N-Acetyl-Galactosaminyl Transferase 4 (B4GALNT4)

Type de proteíne: Recombinant

Attributs du protein: AA 37-1039

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: His tag

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Séquence: RQGRALRQRL GYGRDGEKLT SETDGRGVHA APSTQRAEDS SESREEEQAP EGRDLDMLFP GGAGRLPLNF THQTPPWREE YKGQVNLHVF EDWCGGAVGH LRRNLHFPLF PHTRTTVKKL AVSPKWKNYG LRIFGFIHPA RDGDVQFSVA SDDNSEFWLS LDESPAAAQL VAFVGKTGSE WTAPGEFTKF SSQVSKPRRL MASRRYYFEL LHKQDDRGSD HVEVGWRAFL PGLKFEVISS AHISLYTDES ALKMDHVAHV PQSPASHVGG RPPQEETSAD MLRPDPRDTF FLTPRMESSS LENVLEPCAY APTYVVKDFP IARYQGLQFV YLSFVYPNDY TRLTHMETDN KCFYRESPLY LERFGFYKYM KMDKEEGDED EEDEVQRRAF LFLNPDDFLD DEDEGELLDS LEPTEAAPPR SGPQSPAPAA PAQPGATLAP PTPPRPRDGG TPRHSRALSW AARAARPLPL FLGRAPPPRP AVEQPPPKVY VTRVRPGQRA SPRAPAPRAP WPPFPGVFLH PRPLPRVQLR APPRPPRPHG RRTGGPQATQ PRPPARAQAT QGGREGQART LGPAAPTVDS NLSSEARPVT SFLSLSQVSG PQLPGEGEEE EEGEDDGAPG DEAASEDSEE AAGPALGRWR EDAIDWQRTF SVGAVDFELL RSDWNDLRCN VSGNLQLPEA EAVDVTAQYM ERLNARHGGR FALLRIVNVE KRRDSARGSR FLLELELQER GGGRLRLSEY VFLRLPGARV GDADGESPEP APAASVRPDG RPELCRPLRL AWRQDVMVHF IVPVKNQARW VAQFLADMAA LHARTGDSRF SVVLVDFESE DMDVERALRA ARLPRYQYLR RTGNFERSAG LQAGVDAVED ASSIVFLCDL HIHFPPNILD GIRKHCVEGR LAFAPVVMRL SCGSSPRDPH GYWEVNGFGL FGIYKSDFDR VGGMNTEEFR DQWGGEDWEL LDRVLQAGLE VERLRLRNFY HHYHSKRGMW SVRSRKGSRT GAS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human B4GALNT4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: beta-1,4-N-Acetyl-Galactosaminyl Transferase 4 (B4GALNT4)

Autre désignation: B4GALNT4 (B4GALNT4 Produits)

Synonymes: BC038881 Protein, Gm1095 Protein, beta4GalNAcT4 Protein, beta-1,4-N-acetyl-galactosaminyltransferase 4 Protein, beta-1,4-N-acetyl-galactosaminyl transferase 4 Protein, B4GALNT4 Protein, B4galnt4 Protein

Sujet: Transfers N-acetylgalactosamine (GalNAc) from UDP-GalNAc to N-acetylglucosamine-beta-benzyl with a beta-1,4-linkage to form N,N'-diacetyllactosediamine, GalNAc-beta-1,4-GlcNAc structures in N-linked glycans and probably O-linked glycans.

Poids moléculaire: 113.3 kDa Including tag.

UniProt: Q76KP1