Cadherin 12 Protein (AA 55-609) (MBP tag)

N° du produit ABIN3090213

Détail du produit

Antigène: Cadherin 12

Type de proteíne: Recombinant

Attributs du protein: AA 55-609

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette Cadherin 12 protéine est marqué à la MBP tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: GWVWNQFFVL EEYVGSEPQY VGKLHSDLDK GEGTVKYTLS GDGAGTVFTI DETTGDIHAI RSLDREEKPF YTLRAQAVDI ETRKPLEPES EFIIKVQDIN DNEPKFLDGP YVATVPEMSP VGAYVLQVKA TDADDPTYGN SARVVYSILQ GQPYFSIDPK TGVIRTALPN MDREVKEQYQ VLIQAKDMGG QLGGLAGTTI VNITLTDVND NPPRFPKSIF HLKVPESSPI GSAIGRIRAV DPDFGQNAEI EYNIVPGDGG NLFDIVTDED TQEGVIKLKK PLDFETKKAY TFKVEASNLH LDHRFHSAGP FKDTATVKIS VLDVDEPPVF SKPLYTMEVY EDTPVGTIIG AVTAQDLDVG SSAVRYFIDW KSDGDSYFTI DGNEGTIATN ELLDRESTAQ YNFSIIASKV SNPLLTSKVN ILINVLDVNE FPPEISVPYE TAVCENAKPG QIIQIVSAAD RDLSPAGQQF SFRLSPEAAI KPNFTVRDFR NNTAGIETRR NGYSRRQQEL YFLPVVIEDS SYPVQSSTNT MTIRVCRCDS DGTILSCNVE AIFLP
Sequence without tag.

Specificité: N-terminal MBP-tag

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human CDH12 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

N-terminal MBP-Tag

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: in solution (20 mM Hepes, pH 7.4, 5 mM NaCl)

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Cadherin 12

Autre désignation: CDH12 (Cadherin 12 Produits)

Sujet: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types.

Poids moléculaire: 62.3 kDa Including tag.

UniProt: P55289