CBX3 Protein (AA 2-183) (His tag)

N° du produit ABIN3090507

Détail du produit

Antigène: CBX3 (Chromobox Homolog 3 (CBX3))

Type de proteíne: Recombinant

Attributs du protein: AA 2-183

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette CBX3 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: ASNKTTLQKM GKKQNGKSKK VEEAEPEEFV VEKVLDRRVV NGKVEYFLKW KGFTDADNTW EPEENLDCPE LIEAFLNSQK AGKEKDGTKR KSLSDSESDD SKSKKKRDAA DKPRGFARGL DPERIIGATD SSGELMFLMK WKDSDEADLV LAKEANMKCP QIVIAFYEER LTWHSCPEDE AQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human CBX3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CBX3 (Chromobox Homolog 3 (CBX3))

Autre désignation: CBX3 (CBX3 Produits)

Synonymes: HECH Protein, HP1-GAMMA Protein, HP1Hs-gamma Protein, Hp1-gamma Protein, HP1g Protein, M32 Protein, cbx3 Protein, cbx3a Protein, xhp1g Protein, wu:fb61b02 Protein, zgc:153397 Protein, chromobox 3 Protein, chromobox 3 L homeolog Protein, chromobox protein homolog 3 pseudogene Protein, chromobox homolog 3a (HP1 gamma homolog, Drosophila) Protein, CBX3 Protein, Cbx3 Protein, cbx3.L Protein, LOC100355282 Protein, cbx3a Protein

Sujet: Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1.

Poids moléculaire: 21.6 kDa Including tag.

UniProt: Q13185