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CACNA2D1 Protein (AA 25-1073) (His tag)

Crystallography grade CACNA2D1 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, Crys, ELISA, WB
N° du produit ABIN3090543
  • Antigène Voir toutes CACNA2D1 Protéines
    CACNA2D1 (Calcium Channel, Voltage-Dependent, alpha 2/delta Subunit 1 (CACNA2D1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 25-1073
    Origine
    • 4
    • 4
    Humain
    Source
    • 7
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette CACNA2D1 protéine est marqué à la His tag.
    Application
    SDS-PAGE (SDS), Crystallization (Crys), ELISA, Western Blotting (WB)
    Séquence
    EPFPSAVTIK SWVDKMQEDL VTLAKTASGV NQLVDIYEKY QDLYTVEPNN ARQLVEIAAR DIEKLLSNRS KALVRLALEA EKVQAAHQWR EDFASNEVVY YNAKDDLDPE KNDSEPGSQR IKPVFIEDAN FGRQISYQHA AVHIPTDIYE GSTIVLNELN WTSALDEVFK KNREEDPSLL WQVFGSATGL ARYYPASPWV DNSRTPNKID LYDVRRRPWY IQGAASPKDM LILVDVSGSV SGLTLKLIRT SVSEMLETLS DDDFVNVASF NSNAQDVSCF QHLVQANVRN KKVLKDAVNN ITAKGITDYK KGFSFAFEQL LNYNVSRANC NKIIMLFTDG GEERAQEIFN KYNKDKKVRV FTFSVGQHNY DRGPIQWMAC ENKGYYYEIP SIGAIRINTQ EYLDVLGRPM VLAGDKAKQV QWTNVYLDAL ELGLVITGTL PVFNITGQFE NKTNLKNQLI LGVMGVDVSL EDIKRLTPRF TLCPNGYYFA IDPNGYVLLH PNLQPKPIGV GIPTINLRKR RPNIQNPKSQ EPVTLDFLDA ELENDIKVEI RNKMIDGESG EKTFRTLVKS QDERYIDKGN RTYTWTPVNG TDYSLALVLP TYSFYYIKAK LEETITQARY SETLKPDNFE ESGYTFIAPR DYCNDLKISD NNTEFLLNFN EFIDRKTPNN PSCNADLINR VLLDAGFTNE LVQNYWSKQK NIKGVKARFV VTDGGITRVY PKEAGENWQE NPETYEDSFY KRSLDNDNYV FTAPYFNKSG PGAYESGIMV SKAVEIYIQG KLLKPAVVGI KIDVNSWIEN FTKTSIRDPC AGPVCDCKRN SDVMDCVILD DGGFLLMANH DDYTNQIGRF FGEIDPSLMR HLVNISVYAF NKSYDYQSVC EPGAAPKQGA GHRSAYVPSV ADILQIGWWA TAAAWSILQQ FLLSLTFPRL LEAVEMEDDD FTASLSKQSC ITEQTQYFFD NDSKSFSGVL DCGNCSRIFH GEKLMNTNLI FIMVESKGTC PCDTRLLIQA EQTSDGPNPC DMVKQPRYRK GPDVCFDNNV LEDYTDCGG
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human CACNA2D1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product CACNA2D1 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    CACNA2D1 (Calcium Channel, Voltage-Dependent, alpha 2/delta Subunit 1 (CACNA2D1))
    Autre désignation
    CACNA2D1 (CACNA2D1 Produits)
    Synonymes
    CACNA2D1 Protein, DKFZp469K2211 Protein, CACNA2 Protein, CACNL2A Protein, CCHL2A Protein, Ca(v)alpha2delta1 Protein, Cacna2 Protein, Cchl2a Protein, CCHLA2 Protein, DHSCCA Protein, calcium voltage-gated channel auxiliary subunit alpha2delta 1 Protein, calcium channel, voltage-dependent, alpha 2/delta subunit 1 Protein, calcium channel, voltage-dependent, alpha2/delta subunit 1 Protein, CACNA2D1 Protein, cacna2d1 Protein, Cacna2d1 Protein
    Sujet
    The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Plays an important role in excitation-contraction coupling (By similarity). {ECO:0000250}.
    Poids moléculaire
    119.7 kDa Including tag.
    UniProt
    P54289
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