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CAPZB Protein (AA 2-277) (His tag)

Crystallography grade CAPZB Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3090647
  • Antigène Voir toutes CAPZB Protéines
    CAPZB (Capping Protein (Actin Filament) Muscle Z-Line, beta (CAPZB))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-277
    Origine
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Source
    • 5
    • 2
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette CAPZB protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    SDQQLDCALD LMRRLPPQQI EKNLSDLIDL VPSLCEDLLS SVDQPLKIAR DKVVGKDYLL CDYNRDGDSY RSPWSNKYDP PLEDGAMPSA RLRKLEVEAN NAFDQYRDLY FEGGVSSVYL WDLDHGFAGV ILIKKAGDGS KKIKGCWDSI HVVEVQEKSS GRTAHYKLTS TVMLWLQTNK SGSGTMNLGG SLTRQMEKDE TVSDCSPHIA NIGRLVEDME NKIRSTLNEI YFGKTKDIVN GLRSIDAIPD NQKFKQLQRE LSQVLTQRQI YIQPDN
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human CAPZB Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product CAPZB Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    CAPZB (Capping Protein (Actin Filament) Muscle Z-Line, beta (CAPZB))
    Autre désignation
    CAPZB (CAPZB Produits)
    Synonymes
    CAPB Protein, CAPPB Protein, CAPZ Protein, wu:fk65f06 Protein, zgc:66149 Protein, MGC52754 Protein, capzb Protein, 1700120C01Rik Protein, AI325129 Protein, CPB1 Protein, CPB2 Protein, CPbeat2 Protein, CPbeta1 Protein, CPbeta2 Protein, Cappb1 Protein, capping actin protein of muscle Z-line beta subunit Protein, capping protein (actin filament) muscle Z-line, beta Protein, F-actin-capping protein subunit beta Protein, f-actin-capping protein subunit beta Protein, capping protein (actin filament) muscle Z-line, beta L homeolog Protein, CAPZB Protein, capzb Protein, AOR_1_1412174 Protein, MCYG_05913 Protein, VDBG_04956 Protein, MGYG_06053 Protein, PGTG_03178 Protein, Tsp_07516 Protein, capzb.L Protein, Capzb Protein
    Sujet
    F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Plays a role in the regulation of cell morphology and cytoskeletal organization. {ECO:0000269|PubMed:21834987}.
    Poids moléculaire
    32.2 kDa Including tag.
    UniProt
    P47756
    Pathways
    Regulation of Actin Filament Polymerization
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