CDON Protein (AA 26-963) (His tag)

N° du produit ABIN3090900

Détail du produit

Antigène: CDON (Cell Adhesion Molecule-Related/down-Regulated By Oncogenes (CDON))

Type de proteíne: Recombinant

Attributs du protein: AA 26-963

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette CDON protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: DLAPYFTSEP LSAVQKLGGP VVLHCSAQPV TTRISWLHNG KTLDGNLEHV KIHQGTLTIL SLNSSLLGYY QCLANNSIGA IVSGPATVSV AVLGDFGSST KHVITAEEKS AGFIGCRVPE SNPKAEVRYK IRGKWLEHST ENYLILPSGN LQILNVSLED KGSYKCAAYN PVTHQLKVEP IGRKLLVSRP SSDDVHILHP THSQALAVLS RSPVTLECVV SGVPAPQVYW LKDGQDIAPG SNWRRLYSHL ATDSVDPADS GNYSCMAGNK SGDVKYVTYM VNVLEHASIS KGLQDQIVSL GATVHFTCDV HGNPAPNCTW FHNAQPIHPS ARHLTAGNGL KISGVTVEDV GMYQCVADNG IGFMHSTGRL EIENDGGFKP VIITAPVSAK VADGDFVTLS CNASGLPVPV IRWYDSHGLI TSHPSQVLRS KSRKSQLSRP EGLNLEPVYF VLSQAGASSL HIQAVTQEHA GKYICEAANE HGTTQAEASL MVVPFETNTK AETVTLPDAA QNDDRSKRDG SETGLLSSFP VKVHPSAVES APEKNASGIS VPDAPIILSP PQTHTPDTYN LVWRAGKDGG LPINAYFVKY RKLDDGVGML GSWHTVRVPG SENELHLAEL EPSSLYEVLM VARSAAGEGQ PAMLTFRTSK EKTASSKNTQ ASSPPVGIPK YPVVSEAANN NFGVVLTDSS RHSGVPEAPD RPTISTASET SVYVTWIPRA NGGSPITAFK VEYKRMRTSN WLVAAEDIPP SKLSVEVRSL EPGSTYKFRV IAINHYGESF RSSASRPYQV VGFPNRFSSR PITGPHIAYT EAVSDTQIML KWTYIPSSNN NTPIQGFYIY YRPTDSDNDS DYKRDVVEGS KQWHMIGHLQ PETSYDIKMQ CFNEGGESEF SNVMICETKV KRVPGASEYP VKDLSTPPNS LGSGGNVGPA TSPARSSD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human CDON Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CDON (Cell Adhesion Molecule-Related/down-Regulated By Oncogenes (CDON))

Autre désignation: CDON (CDON Produits)

Synonymes: CDO Protein, CDON1 Protein, HPE11 Protein, ORCAM Protein, Cdo Protein, Cd0 Protein, Orcam Protein, cb507 Protein, wu:fa10c03 Protein, wu:fb94b11 Protein, zgc:158240 Protein, cdon-A Protein, cell adhesion associated, oncogene regulated Protein, cell adhesion molecule-related/down-regulated by oncogenes Protein, cell adhesion associated, oncogene regulated L homeolog Protein, CDON Protein, Cdon Protein, cdon Protein, cdon.L Protein

Sujet: Component of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells. Promotes differentiation of myogenic cells (By similarity). {ECO:0000250}.

Poids moléculaire: 102.3 kDa Including tag.

UniProt: Q4KMG0

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Embryonic Body Morphogenesis