Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

C5 Protein (AA 752-1676) (His tag)

Crystallography grade C5 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N° du produit ABIN3091806
  • Antigène Voir toutes C5 Protéines
    C5 (Complement Component 5 (C5))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 752-1676
    Origine
    • 8
    • 7
    • 4
    • 1
    • 1
    • 1
    • 1
    Humain
    Source
    • 7
    • 7
    • 5
    • 3
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette C5 protéine est marqué à la His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Séquence
    LHMKTLLPVS KPEIRSYFPE SWLWEVHLVP RRKQLQFALP DSLTTWEIQG VGISNTGICV ADTVKAKVFK DVFLEMNIPY SVVRGEQIQL KGTVYNYRTS GMQFCVKMSA VEGICTSESP VIDHQGTKSS KCVRQKVEGS SSHLVTFTVL PLEIGLHNIN FSLETWFGKE ILVKTLRVVP EGVKRESYSG VTLDPRGIYG TISRRKEFPY RIPLDLVPKT EIKRILSVKG LLVGEILSAV LSQEGINILT HLPKGSAEAE LMSVVPVFYV FHYLETGNHW NIFHSDPLIE KQKLKKKLKE GMLSIMSYRN ADYSYSVWKG GSASTWLTAF ALRVLGQVNK YVEQNQNSIC NSLLWLVENY QLDNGSFKEN SQYQPIKLQG TLPVEARENS LYLTAFTVIG IRKAFDICPL VKIDTALIKA DNFLLENTLP AQSTFTLAIS AYALSLGDKT HPQFRSIVSA LKREALVKGN PPIYRFWKDN LQHKDSSVPN TGTARMVETT AYALLTSLNL KDINYVNPVI KWLSEEQRYG GGFYSTQDTI NAIEGLTEYS LLVKQLRLSM DIDVSYKHKG ALHNYKMTDK NFLGRPVEVL LNDDLIVSTG FGSGLATVHV TTVVHKTSTS EEVCSFYLKI DTQDIEASHY RGYGNSDYKR IVACASYKPS REESSSGSSH AVMDISLPTG ISANEEDLKA LVEGVDQLFT DYQIKDGHVI LQLNSIPSSD FLCVRFRIFE LFEVGFLSPA TFTVYEYHRP DKQCTMFYST SNIKIQKVCE GAACKCVEAD CGQMQEELDL TISAETRKQT ACKPEIAYAY KVSITSITVE NVFVKYKATL LDIYKTGEAV AEKDSEITFI KKVTCTNAEL VKGRQYLIMG KEALQIKYNF SFRYIYPLDS LTWIEYWPRD TTCSSCQAFL ANLDEFAEDI FLNGC
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human C5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product C5 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    C5 (Complement Component 5 (C5))
    Autre désignation
    C5 (C5 Produits)
    Synonymes
    C5 Protein, LOC565774 Protein, C5a Protein, C5b Protein, CPAMD4 Protein, Hc Protein, RGD1561905 Protein, He Protein, complement C5 Protein, complement component 5 Protein, hemolytic complement Protein, C5 Protein, c5 Protein, Hc Protein
    Sujet
    Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled., Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed:8182049). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation. {ECO:0000269|PubMed:8182049}.
    Poids moléculaire
    105.2 kDa Including tag.
    UniProt
    P01031
    Pathways
    Système du Complément, Carbohydrate Homeostasis
Vous êtes ici:
Support technique