Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

CRTC3 Protein (AA 1-619) (His tag)

Crystallography grade CRTC3 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N° du produit ABIN3091901
  • Antigène Voir toutes CRTC3 Protéines
    CRTC3 (CREB Regulated Transcription Coactivator 3 (CRTC3))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-619
    Origine
    • 2
    • 1
    Humain
    Source
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette CRTC3 protéine est marqué à la His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Séquence
    MAASPGSGSA NPRKFSEKIA LHTQRQAEET RAFEQLMTDL TLSRVQFQKL QQLRLTQYHG GSLPNVSQLR SSASEFQPSF HQADNVRGTR HHGLVERPSR NRFHPLHRRS GDKPGRQFDG SAFGANYSSQ PLDESWPRQQ PPWKDEKHPG FRLTSALNRT NSDSALHTSA LSTKPQDPYG GGGQSAWPAP YMGFCDGENN GHGEVASFPG PLKEENLLNV PKPLPKQLWE TKEIQSLSGR PRSCDVGGGN AFPHNGQNLG LSPFLGTLNT GGSLPDLTNL HYSTPLPASL DTTDHHFGSM SVGNSVNNIP AAMTHLGIRS SSGLQSSRSN PSIQATLNKT VLSSSLNNHP QTSVPNASAL HPSLRLFSLS NPSLSTTNLS GPSRRRQPPV SPLTLSPGPE AHQGFSRQLS STSPLAPYPT SQMVSSDRSQ LSFLPTEAQA QVSPPPPYPA PQELTQPLLQ QPRAPEAPAQ QPQAASSLPQ SDFQLLPAQG SSLTNFFPDV GFDQQSMRPG PAFPQQVPLV QQGSRELQDS FHLRPSPYSN CGSLPNTILP EDSSTSLFKD LNSALAGLPE VSLNVDTPFP LEEELQIEPL SLDGLNMLSD SSMGLLDPSV EETFRADRL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human CRTC3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product CRTC3 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    CRTC3 (CREB Regulated Transcription Coactivator 3 (CRTC3))
    Autre désignation
    CRTC3 (CRTC3 Produits)
    Synonymes
    torc3 Protein, MGC146372 Protein, 2610312F20Rik Protein, 6332415K15Rik Protein, AI429792 Protein, BC011210 Protein, TORC3 Protein, RGD1309666 Protein, TORC-3 Protein, CREB regulated transcription coactivator 3 Protein, CREB regulated transcription coactivator 3 L homeolog Protein, CRTC3 Protein, crtc3 Protein, Crtc3 Protein, crtc3.L Protein
    Sujet
    Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). {ECO:0000269|PubMed:14506290, ECO:0000269|PubMed:15454081, ECO:0000269|PubMed:15466468, ECO:0000269|PubMed:16817901, ECO:0000269|PubMed:16980408, ECO:0000269|PubMed:17210223, ECO:0000269|PubMed:17644518}.
    Poids moléculaire
    67.9 kDa Including tag.
    UniProt
    Q6UUV7
    Pathways
    SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2
Vous êtes ici:
Support technique