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COPB2 Protein (AA 2-906) (His tag)

Crystallography grade COPB2 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3091936
  • Antigène Voir toutes COPB2 Protéines
    COPB2 (Coatomer Protein Complex, Subunit beta 2 (COPB2))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-906
    Origine
    • 3
    • 1
    Humain
    Source
    • 2
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette COPB2 protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    PLRLDIKRKL TARSDRVKSV DLHPTEPWML ASLYNGSVCV WNHETQTLVK TFEVCDLPVR AAKFVARKNW VVTGADDMQI RVFNYNTLER VHMFEAHSDY IRCIAVHPTQ PFILTSSDDM LIKLWDWDKK WSCSQVFEGH THYVMQIVIN PKDNNQFASA SLDRTIKVWQ LGSSSPNFTL EGHEKGVNCI DYYSGGDKPY LISGADDRLV KIWDYQNKTC VQTLEGHAQN VSCASFHPEL PIIITGSEDG TVRIWHSSTY RLESTLNYGM ERVWCVASLR GSNNVALGYD EGSIIVKLGR EEPAMSMDAN GKIIWAKHSE VQQANLKAMG DAEIKDGERL PLAVKDMGSC EIYPQTIQHN PNGRFVVVCG DGEYIIYTAM ALRNKSFGSA QEFAWAHDSS EYAIRESNSI VKIFKNFKEK KSFKPDFGAE SIYGGFLLGV RSVNGLAFYD WDNTELIRRI EIQPKHIFWS DSGELVCIAT EESFFILKYL SEKVLAAQET HEGVTEDGIE DAFEVLGEIQ EIVKTGLWVG DCFIYTSSVN RLNYYVGGEI VTIAHLDRTM YLLGYIPKDN RLYLGDKELN IISYSLLVSV LEYQTAVMRR DFSMADKVLP TIPKEQRTRV AHFLEKQGFK QQALTVSTDP EHRFELALQL GELKIAYQLA VEAESEQKWK QLAELAISKC QFGLAQECLH HAQDYGGLLL LATASGNANM VNKLAEGAER DGKNNVAFMS YFLQGKVDAC LELLIRTGRL PEAAFLARTY LPSQVSRVVK LWRENLSKVN QKAAESLADP TEYENLFPGL KEAFVVEEWV KETHADLWPA KQYPLVTPNE ERNVMEEGKD FQPSRSTAQQ ELDGKPASPT PVIVASHTAN KEEKSLLELE VDLDNLELED IDTTDINLDE DILDD
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human COPB2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product COPB2 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    COPB2 (Coatomer Protein Complex, Subunit beta 2 (COPB2))
    Autre désignation
    COPB2 (COPB2 Produits)
    Synonymes
    beta'-COP Protein, sb:cb121 Protein, wu:fb30f06 Protein, AI256832 Protein, copb2 Protein, copb2.L Protein, wu:fc55e05 Protein, Beta'-COP Protein, coatomer protein complex subunit beta 2 Protein, coatomer beta subunit Protein, coatomer protein complex, subunit beta 2 Protein, coatomer beta' subunit Protein, Coatomer beta' subunit Protein, coatomer protein complex, subunit beta 2 (beta prime) Protein, coatomer protein complex subunit beta 2 (beta prime) S homeolog Protein, COPB2 Protein, beta-coP Protein, copb2 Protein, NCU07319 Protein, ATEG_01512 Protein, LELG_04874 Protein, HCAG_08724 Protein, GL50803_9593 Protein, CTRG_02922 Protein, UREG_02221 Protein, EBI_25283 Protein, Copb2 Protein, copb2.S Protein
    Sujet
    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins, the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). {ECO:0000250}., This coatomer complex protein, essential for Golgi budding and vesicular trafficking, is a selective binding protein (RACK) for protein kinase C, epsilon type. It binds to Golgi membranes in a GTP-dependent manner (By similarity). Interacts with JAGN1. {ECO:0000250, ECO:0000269|PubMed:25129144}.
    Poids moléculaire
    103.3 kDa Including tag.
    UniProt
    P35606
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