CTNND2 Protein (AA 1-1225) (Strep Tag)

N° du produit ABIN3092019

Détail du produit

Antigène: CTNND2 (Catenin (Cadherin-Associated Protein), delta 2 (Neural Plakophilin-Related Arm-Repeat Protein (CTNND2))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1225

Origine: Humain

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette CTNND2 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MFARKPPGAA PLGAMPVPDQ PSSASEKTSS LSPGLNTSNG DGSETETTSA ILASVKEQEL QFERLTRELE AERQIVASQL ERCKLGSETG SMSSMSSAEE QFQWQSQDGQ KDIEDELTTG LELVDSCIRS LQESGILDPQ DYSTGERPSL LSQSALQLNS KPEGSFQYPA SYHSNQTLAL GETTPSQLPA RGTQARATGQ SFSQGTTSRA GHLAGPEPAP PPPPPPREPF APSLGSAFHL PDAPPAAAAA ALYYSSSTLP APPRGGSPLA APQGGSPTKL QRGGSAPEGA TYAAPRGSSP KQSPSRLAKS YSTSSPINIV VSSAGLSPIR VTSPPTVQST ISSSPIHQLS STIGTYATLS PTKRLVHASE QYSKHSQELY ATATLQRPGS LAAGSRASYS SQHGHLGPEL RALQSPEHHI DPIYEDRVYQ KPPMRSLSQS QGDPLPPAHT GTYRTSTAPS SPGVDSVPLQ RTGSQHGPQN AAAATFQRAS YAAGPASNYA DPYRQLQYCP SVESPYSKSG PALPPEGTLA RSPSIDSIQK DPREFGWRDP ELPEVIQMLQ HQFPSVQSNA AAYLQHLCFG DNKIKAEIRR QGGIQLLVDL LDHRMTEVHR SACGALRNLV YGKANDDNKI ALKNCGGIPA LVRLLRKTTD LEIRELVTGV LWNLSSCDAL KMPIIQDALA VLTNAVIIPH SGWENSPLQD DRKIQLHSSQ VLRNATGCLR NVSSAGEEAR RRMRECDGLT DALLYVIQSA LGSSEIDSKT VENCVCILRN LSYRLAAETS QGQHMGTDEL DGLLCGEANG KDAESSGCWG KKKKKKKSQD QWDGVGPLPD CAEPPKGIQM LWHPSIVKPY LTLLSECSNP DTLEGAAGAL QNLAAGSWKW SVYIRAAVRK EKGLPILVEL LRIDNDRVVC AVATALRNMA LDVRNKELIG KYAMRDLVHR LPGGNNSNNT ASKAMSDDTV TAVCCTLHEV ITKNMENAKA LRDAGGIEKL VGISKSKGDK HSPKVVKAAS QVLNSMWQYR DLRSLYKKDG WSQYHFVASS STIERDRQRP YSSSRTPSIS PVRVSPNNRS ASAPASPREM ISLKERKTDY ECTGSNATYH GAKGEHTSRK DAMTAQNTGI STLYRNSYGA PAEDIKHNQV SAQPVPQEPS RKDYETYQPF QNSTRNYDES FFEDQVHHRP PASEYTMHLG LKSTGNYVDF YSAARPYSEL NYETSHYPAS PDSWV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CTNND2 (Catenin (Cadherin-Associated Protein), delta 2 (Neural Plakophilin-Related Arm-Repeat Protein (CTNND2))

Autre désignation: CTNND2 (CTNND2 Produits)

Synonymes: CTNND2 Protein, Catnd2 Protein, Nprap Protein, neurojugin Protein, GT24 Protein, NPRAP Protein, ctnnd2 Protein, si:dkey-40c17.4 Protein, catenin delta 2 Protein, delta-catenin Protein, catenin delta-2 Protein, catenin (cadherin associated protein), delta 2 Protein, catenin (cadherin-associated protein), delta 2b Protein, CTNND2 Protein, BACOIKO001_27 Protein, LOC100070792 Protein, Ctnnd2 Protein, ctnnd2b Protein

Sujet: Catenin delta-2 (Delta-catenin) (GT24) (Neural plakophilin-related ARM-repeat protein) (NPRAP) (Neurojungin),FUNCTION: Has a critical role in neuronal development, particularly in the formation and/or maintenance of dendritic spines and synapses (PubMed:25807484). Involved in the regulation of Wnt signaling (PubMed:25807484). It probably acts on beta-catenin turnover, facilitating beta-catenin interaction with GSK3B, phosphorylation, ubiquitination and degradation (By similarity). Functions as a transcriptional activator when bound to ZBTB33 (By similarity). May be involved in neuronal cell adhesion and tissue morphogenesis and integrity by regulating adhesion molecules. {ECO:0000250|UniProtKB:O35927, ECO:0000269|PubMed:25807484, ECO:0000269|PubMed:9971746}.

Poids moléculaire: 132.7 kDa

UniProt: Q9UQB3