DPYD Protein (AA 4-1025) (His tag)
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- Antigène Voir toutes DPYD Protéines
- DPYD (Dihydropyrimidine Dehydrogenase (DPYD))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 4-1025
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Origine
- Humain
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette DPYD protéine est marqué à la His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Séquence
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VLSKDSADIE SILALNPRTQ THATLCSTSA KKLDKKHWKR NPDKNCFNCE KLENNFDDIK HTTLGERGAL REAMRCLKCA DAPCQKSCPT NLDIKSFITS IANKNYYGAA KMIFSDNPLG LTCGMVCPTS DLCVGGCNLY ATEEGPINIG GLQQFATEVF KAMSIPQIRN PSLPPPEKMS EAYSAKIALF GAGPASISCA SFLARLGYSD ITIFEKQEYV GGLSTSEIPQ FRLPYDVVNF EIELMKDLGV KIICGKSLSV NEMTLSTLKE KGYKAAFIGI GLPEPNKDAI FQGLTQDQGF YTSKDFLPLV AKGSKAGMCA CHSPLPSIRG VVIVLGAGDT AFDCATSALR CGARRVFIVF RKGFVNIRAV PEEMELAKEE KCEFLPFLSP RKVIVKGGRI VAMQFVRTEQ DETGKWNEDE DQMVHLKADV VISAFGSVLS DPKVKEALSP IKFNRWGLPE VDPETMQTSE AWVFAGGDVV GLANTTVESV NDGKQASWYI HKYVQSQYGA SVSAKPELPL FYTPIDLVDI SVEMAGLKFI NPFGLASATP ATSTSMIRRA FEAGWGFALT KTFSLDKDIV TNVSPRIIRG TTSGPMYGPG QSSFLNIELI SEKTAAYWCQ SVTELKADFP DNIVIASIMC SYNKNDWTEL AKKSEDSGAD ALELNLSCPH GMGERGMGLA CGQDPELVRN ICRWVRQAVQ IPFFAKLTPN VTDIVSIARA AKEGGANGVT ATNTVSGLMG LKSDGTPWPA VGIAKRTTYG GVSGTAIRPI ALRAVTSIAR ALPGFPILAT GGIDSAESGL QFLHSGASVL QVCSAIQNQD FTVIEDYCTG LKALLYLKSI EELQDWDGQS PATVSHQKGK PVPRIAELMD KKLPSFGPYL EQRKKIIAEN KIRLKEQNVA FSPLKRNCFI PKRPIPTIKD VIGKALQYLG TFGELSNVEQ VVAMIDEEMC INCGKCYMTC NDSGYQAIQF DPETHLPTIT DTCTGCTLCL SVCPIVDCIK MVSRTTPYEP KRGVPLSVNP VC
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human DPYD Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product DPYD Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- DPYD (Dihydropyrimidine Dehydrogenase (DPYD))
- Autre désignation
- DPYD (DPYD Produits)
- Synonymes
- DPYD Protein, dpyd Protein, zgc:77205 Protein, AI315208 Protein, DPD Protein, E330028L06Rik Protein, DHP Protein, DHPDHASE Protein, dhp Protein, dhpdhase Protein, dpd Protein, 14.t00017 Protein, DDBDRAFT_0189681 Protein, DDBDRAFT_0231100 Protein, DDB_0189681 Protein, DDB_0231100 Protein, dpyda Protein, zgc:153505 Protein, dihydropyrimidine dehydrogenase Protein, dihydropyrimidine dehydrogenase b Protein, dihydropyrimidine dehydrogenase L homeolog Protein, dihydropyrimidine dehydrogenase [NADP(+)] Protein, dihydropyrimidine dehydrogenase a, tandem duplicate 1 Protein, DPYD Protein, Dpyd Protein, dpydb Protein, dpyd.L Protein, EHI_012980 Protein, LOC5573038 Protein, EDI_044620 Protein, pyd1 Protein, dpyda.1 Protein
- Sujet
- Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil.
- Poids moléculaire
- 112.1 kDa Including tag.
- UniProt
- Q12882
- Pathways
- Ribonucleoside Biosynthetic Process
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