FAM65B Protein (AA 1-1068) (His tag)

N° du produit ABIN3092486

Détail du produit

Antigène: FAM65B (Family with Sequence Similarity 65, Member B (FAM65B))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1068

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette FAM65B protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: MLVGSQSFSP GGPNGIIRSQ SFAGFSGLQE RRSRCNSFIE NSSALKKPQA KLKKMHNLGH KNNNPPKEPQ PKRVEEVYRA LKNGLDEYLE VHQTELDKLT AQLKDMKRNS RLGVLYDLDK QIKTIERYMR RLEFHISKVD ELYEAYCIQR RLQDGASKMK QAFATSPASK AARESLTEIN RSFKEYTENM CTIEVELENL LGEFSIKMKG LAGFARLCPG DQYEIFMKYG RQRWKLKGKI EVNGKQSWDG EETVFLPLIV GFISIKVTEL KGLATHILVG SVTCETKELF AARPQVVAVD INDLGTIKLN LEITWYPFDV EDMTASSGAG NKAAALQRRM SMYSQGTPET PTFKDHSFFR WLHPSPDKPR RLSVLSALQD TFFAKLHRSR SFSDLPSLRP SPKAVLELYS NLPDDIFENG KAAEEKMPLS LSFSDLPNGD CALTSHSTGS PSNSTNPEIT ITPAEFNLSS LASQNEGMDD TSSASSRNSL GEGQEPKSHL KEEDPEEPRK PASAPSEACR RQSSGAGAEH LFLENDVAEA LLQESEEASE LKPVELDTSE GNITKQLVKR LTSAEVPMAT DRLLSEGSVG GESEGCRSFL DGSLEDAFNG LLLALEPHKE QYKEFQDLNQ EVMNLDDILK CKPAVSRSRS SSLSLTVESA LESFDFLNTS DFDEEEDGDE VCNVGGGADS VFSDTETEKH SYRSVHPEAR GHLSEALTED TGVGTSVAGS PLPLTTGNES LDITIVRHLQ YCTQLVQQIV FSSKTPFVAR SLLEKLSRQI QVMEKLAAVS DENIGNISSV VEAIPEFHKK LSLLSFWTKC CSPVGVYHSP ADRVMKQLEA SFARTVNKEY PGLADPVFRT LVSQILDRAE PLLSSSLSSE VVTVFQYYSY FTSHGVSDLE SYLSQLARQV SMVQTLQSLR DEKLLQTMSD LAPSNLLAQQ EVLRTLALLL TREDNEVSEA VTLYLAAASK NQHFREKALL YYCEALTKTN LQLQKAACLA LKILEATESI KMLVTLCQSD TEEIRNVASE TLLSLGEDGR LAYEQLDKFP RDCVKVGGRH GTEVATAF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human FAM65B Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: FAM65B (Family with Sequence Similarity 65, Member B (FAM65B))

Autre désignation: FAM65B (FAM65B Produits)

Synonymes: C6orf32 Protein, DIFF40 Protein, DIFF48 Protein, MYONAP Protein, PL48 Protein, 1700108N18Rik Protein, 6330500D04Rik Protein, AI225904 Protein, E430013J17Rik Protein, si:dkey-218n20.1 Protein, Ab2-162 Protein, RGD1306939 Protein, RHO family interacting cell polarization regulator 2 Protein, family with sequence similarity 65, member B Protein, ripor2 Protein, RIPOR2 Protein, Ripor2 Protein, FAM65B Protein

Sujet: Required for hearing (PubMed:24958875). Involved in skeletal muscle development (PubMed:24687993). {ECO:0000269|PubMed:24687993, ECO:0000269|PubMed:24958875}., Isoform 2: Plays a role in promoting myogenic cell differentiation, cytoskeletal rearrangement and filopodia formation (PubMed:17150207). {ECO:0000269|PubMed:17150207}.

Poids moléculaire: 119.5 kDa Including tag.

UniProt: Q9Y4F9

Pathways: Transition Metal Ion Homeostasis