ERAP1 Protein (AA 22-941) (His tag)

N° du produit ABIN3092490

Détail du produit

Antigène: ERAP1 (Endoplasmic Reticulum Aminopeptidase 1 (ERAP1))

Type de proteíne: Recombinant

Attributs du protein: AA 22-941

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette ERAP1 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: ALLTVSTPSW CQSTEASPKR SDGTPFPWNK IRLPEYVIPV HYDLLIHANL TTLTFWGTTK VEITASQPTS TIILHSHHLQ ISRATLRKGA GERLSEEPLQ VLEHPRQEQI ALLAPEPLLV GLPYTVVIHY AGNLSETFHG FYKSTYRTKE GELRILASTQ FEPTAARMAF PCFDEPAFKA SFSIKIRREP RHLAISNMPL VKSVTVAEGL IEDHFDVTVK MSTYLVAFII SDFESVSKIT KSGVKVSVYA VPDKINQADY ALDAAVTLLE FYEDYFSIPY PLPKQDLAAI PDFQSGAMEN WGLTTYRESA LLFDAEKSSA SSKLGITMTV AHELAHQWFG NLVTMEWWND LWLNEGFAKF MEFVSVSVTH PELKVGDYFF GKCFDAMEVD ALNSSHPVST PVENPAQIRE MFDDVSYDKG ACILNMLREY LSADAFKSGI VQYLQKHSYK NTKNEDLWDS MASICPTDGV KGMDGFCSRS QHSSSSSHWH QEGVDVKTMM NTWTLQKGFP LITITVRGRN VHMKQEHYMK GSDGAPDTGY LWHVPLTFIT SKSDMVHRFL LKTKTDVLIL PEEVEWIKFN VGMNGYYIVH YEDDGWDSLT GLLKGTHTAV SSNDRASLIN NAFQLVSIGK LSIEKALDLS LYLKHETEIM PVFQGLNELI PMYKLMEKRD MNEVETQFKA FLIRLLRDLI DKQTWTDEGS VSERMLRSQL LLLACVHNYQ PCVQRAEGYF RKWKESNGNL SLPVDVTLAV FAVGAQSTEG WDFLYSKYQF SLSSTEKSQI EFALCRTQNK EKLQWLLDES FKGDKIKTQE FPQILTLIGR NPVGYPLAWQ FLRKNWNKLV QKFELGSSSI AHMVMGTTNQ FSTRTRLEEV KGFFSSLKEN GSQLRCVQQT IETIEENIGW MDKNFDKIRV WLQSEKLERM
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ERAP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ERAP1 (Endoplasmic Reticulum Aminopeptidase 1 (ERAP1))

Autre désignation: ERAP1 (ERAP1 Produits)

Synonymes: MGC166160 Protein, DKFZp469C044 Protein, ARTS-1 Protein, A-LAP Protein, ALAP Protein, APPILS Protein, ARTS1 Protein, ERAAP Protein, ERAAP1 Protein, PILS-AP Protein, PILSAP Protein, Arts1 Protein, PILSA Protein, Appils Protein, endoplasmic reticulum aminopeptidase 1 Protein, endoplasmic reticulum aminopeptidase 1b Protein, ERAP1 Protein, erap1 Protein, erap1b Protein, Erap1 Protein

Sujet: Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney. {ECO:0000269|PubMed:15908954, ECO:0000269|PubMed:16286653, ECO:0000269|PubMed:21478864}.

Poids moléculaire: 105.8 kDa Including tag.

UniProt: Q9NZ08