F-Box Protein 38 (FBXO38) (AA 1-1188) protein (His tag)

Détails pour le produit réf. ABIN3092570, Fournisseur: Connectez-vous pour afficher
Nom de la protéine
  • Fbx38
  • MOKA
  • 6030410I24Rik
  • AU044865
  • AW214031
  • Moka
  • SP329
  • MGC131229
  • F-box protein 38
  • F-box protein 38 L homeolog
  • FBXO38
  • Fbxo38
  • fbxo38.L
  • fbxo38
Attributs du protein
AA 1-1188
1
1
1
Origine
Humain
1
1
Source
Cellules d'insectes
1
1
Type de proteíne
Recombinant
Purification/Conjugué
His tag
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
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Séquence MGPRKKSVKT CIMNNEIPEE MTADETKDYM NQLSHEVLCH IFRYLPLQDI MCMECLSRKL KEAVTLYLRV VRVVDLCAGR WWEYMPSGFT DASFLTLLKK MPDVEQLYGL HPRYLERRRV RGHEAFSIPG VLEALQACPN LVGVETSHLE LVESIWTYMP HVHILGKFRN RNGAFPIPPE NKLKIPIGAK IQTLHLVGVN VPEIPCIPML RHLYMKWVRL TKPQPFKDFL CISLRTFVMR NCAGPTNSLK YVPLVTGLAS ARNLEHLEMV RVPFLGGLIQ HVVEDSWRSG GFRNLHTIVL GACKNALEVD LGYLIITAAR RLHEVRIQPS LTKDGVFSAL KMAELEFPQF ETLHLGYVDE FLLQSRMANA DLVKYGLADV VENPGIITDI GMKAVNEVFS CIKYLAIYNC PHLHNPYNWI SDHSRWTRLV DINLVRCHAL KLDSFGQFIE LLPSLEFISL DQMFREPPKG CARVGLSAGT GIGVSSALVS NQNSNNDDNN AQNNNANIHD NNHHHPDDSD EENDFRQDLQ PGEQQFAADA LNEMEDIVQE DGEVVAESGN NTPAHSQAII PVDVDEEQAG PSGLQRVVKP TSITVHDSES DDEEDSLELQ EVWIPKNGTR RYSEREEKTG ESVQSRELSV SGKGKTPLRK RYNSHQMGQS KQFPLEESSC EKGCQVTSEQ IKADMKAARD IPEKKKNKDV YPSCSSTTAS TVGNSSSHNT ASQSPDFVRT VNSGGSSEPS PTEVDVSRQC ACSPGGSEDS EAMEEGDAES SVCPRCCCHR PQESQRRTSR CSDEERPSTS RACVVNGPDG TRSAFSFRTL PQGGSSGPAH DERTNGSGSG ATGEDRRGSS QPESCDVQSN EDYPRRPLTR ARSRLSHVLL VSESEVAKTK PRHAMKRKRT ADKSTSTSDP VIEDDHVQVL VLKSKNLVGV TMTNCGITDL VLKDCPKMMF IHATRCRVLK HLKVENAPIV NRFDYAQCKK LNMDQVLDQI LRMPPERNRI IYLRPMQQVD TLTLEQKLFS GPYPYHICII HEFSNPPNVR NKVRIRSWMD TIANINQELI KYEFFPEATR SEEDLKKYPK YPWGREIYTL EGVVDGAPYS MISDFPWLRS LRAAEPNSFA RYDFEDDEES TIYAPRRKGQ LSADICMETI GEEISEMRQM KKGVFQRVVA IFIHYCDVNG EPVEDDYI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Attributs du produit
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human FBXO38 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

Purification Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Pureté >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Stérilité 0.22 μm filtered
niveau d'endotoxine Protein is endotoxin free.
Classe de qualité Crystallography grade
Nom de la protéine
Sujet Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. May coactivate KLF7, but does not seem to promote KLF7 ubiquitination (By similarity). {ECO:0000250}.
Poids moléculaire 134.9 kDa Including tag.
UniProt Q6PIJ6
Indications d'application In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Commentaires

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles.
Stock -80 °C
Stockage commentaire Store at -80°C.
Date de péremption Unlimited (if stored properly)
Images (Fournisseur)
 image for F-Box Protein 38 (FBXO38) (AA 1-1188) protein (His tag) (ABIN3092570) „Crystallography Grade“ protein due to multi-step, protein-specific purification process