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GGT1 Protein (AA 27-569) (His tag)

Crystallography grade GGT1 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N° du produit ABIN3092760
  • Antigène Voir toutes GGT1 Protéines
    GGT1 (gamma Glutamyltransferase 1 (GGT1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 27-569
    Origine
    • 9
    • 4
    • 1
    • 1
    Humain
    Source
    • 5
    • 4
    • 3
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette GGT1 protéine est marqué à la His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Séquence
    HHHHHHPSAS KEPDNHVYTR AAVAADAKQC SKIGRDALRD GGSAVDAAIA ALLCVGLMNA HSMGIGGGLF LTIYNSTTRK AEVINAREVA PRLAFATMFN SSEQSQKGGL SVAVPGEIRG YELAHQRHGR LPWARLFQPS IQLARQGFPV GKGLAAALEN KRTVIEQQPV LCEVFCRDRK VLREGERLTL PQLADTYETL AIEGAQAFYN GSLTAQIVKD IQAAGGIVTA EDLNNYRAEL IEHPLNISLG DVVLYMPSAP LSGPVLALIL NILKGYNFSR ESVESPEQKG LTYHRIVEAF RFAYAKRTLL GDPKFVDVTE VVRNMTSEFF AAQLRAQISD DTTHPISYYK PEFYTPDDGG TAHLSVVAED GSAVSATSTI NLYFGSKVRS PVSGILFNNE MDDFSSPSIT NEFGVPPSPA NFIQPGKQPL SSMCPTIMVG QDGQVRMVVG AAGGTQITTA TALAIIYNLW FGYDVKRAVE EPRLHNQLLP NVTTVERNID QAVTAALETR HHHTQIASTF IAVVQAIVRT AGGWAAASDS RKGGEPAGY
    Specificité
    N-terminaler His-Tag
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human GGT1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This made-to-order protein has already been successfully produced. Please let us know if you are interested in purchasing a smaller amount of this protein. We will check our stock and make you a customized quote in case we can provide this protein in a smaller amount..

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    The protein is purified from the cleared cell lysate using DTT resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    150 mM NaCl, 20 mM NaH2PO4, pH 7.4; 10 % Glycerol
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    GGT1 (gamma Glutamyltransferase 1 (GGT1))
    Autre désignation
    GGT1 (GGT1 Produits)
    Synonymes
    ggt1 Protein, zgc:64172 Protein, CD224 Protein, D22S672 Protein, D22S732 Protein, GGT Protein, GGT 1 Protein, GTG Protein, GGT-1 Protein, Ggtp Protein, dwg Protein, GGLUT Protein, Ggt Protein, GGT2 Protein, cd224 Protein, ggt Protein, gtg Protein, GGT1 Protein, ggt1-a Protein, gamma-glutamyltransferase 1a Protein, gamma-glutamyltransferase 1 Protein, gamma-glutamyltranspeptidase 1 Protein, gamma-glutamyltransferase 1 L homeolog Protein, gamma-glutamyltransferase Protein, gamma-glutamyltranspeptidase Ggt1 Protein, ggt1a Protein, GGT1 Protein, Ggt1 Protein, ggt1 Protein, LOC470168 Protein, ggt1.L Protein, ggt Protein, Bcep18194_A6223 Protein, h16_A1098 Protein, Shewana3_2487 Protein, Bcep1808_2978 Protein
    Sujet
    Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates, and other gamma-glutamyl compounds. The metabolism of glutathione releases free glutamate and the dipeptide, cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Isoform 3 seems to be inactive. {ECO:0000269|PubMed:20622017, ECO:0000269|PubMed:24047895, ECO:0000269|PubMed:7673200, ECO:0000269|PubMed:7759490, ECO:0000269|PubMed:8095045, ECO:0000269|PubMed:8827453}.
    Poids moléculaire
    59.5 kDa Including tag.
    UniProt
    P19440
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