GRIK2 Protein (AA 32-908) (rho-1D4 tag)

N° du produit ABIN3092820

Détail du produit

Antigène: GRIK2 (Glutamate Receptor, Ionotropic, Kainate 2 (GRIK2))

Type de proteíne: Recombinant

Attributs du protein: AA 32-908

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette GRIK2 protéine est marqué à la rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: TTHVLRFGGI FEYVESGPMG AEELAFRFAV NTINRNRTLL PNTTLTYDTQ KINLYDSFEA SKKACDQLSL GVAAIFGPSH SSSANAVQSI CNALGVPHIQ TRWKHQVSDN KDSFYVSLYP DFSSLSRAIL DLVQFFKWKT VTVVYDDSTG LIRLQELIKA PSRYNLRLKI RQLPADTKDA KPLLKEMKRG KEFHVIFDCS HEMAAGILKQ ALAMGMMTEY YHYIFTTLDL FALDVEPYRY SGVNMTGFRI LNTENTQVSS IIEKWSMERL QAPPKPDSGL LDGFMTTDAA LMYDAVHVVS VAVQQFPQMT VSSLQCNRHK PWRFGTRFMS LIKEAHWEGL TGRITFNKTN GLRTDFDLDV ISLKEEGLEK IGTWDPASGL NMTESQKGKP ANITDSLSNR SLIVTTILEE PYVLFKKSDK PLYGNDRFEG YCIDLLRELS TILGFTYEIR LVEDGKYGAQ DDANGQWNGM VRELIDHKAD LAVAPLAITY VREKVIDFSK PFMTLGISIL YRKPNGTNPG VFSFLNPLSP DIWMYILLAY LGVSCVLFVI ARFSPYEWYN PHPCNPDSDV VENNFTLLNS FWFGVGALMQ QGSELMPKAL STRIVGGIWW FFTLIIISSY TANLAAFLTV ERMESPIDSA DDLAKQTKIE YGAVEDGATM TFFKKSKIST YDKMWAFMSS RRQSVLVKSN EEGIQRVLTS DYAFLMESTT IEFVTQRNCN LTQIGGLIDS KGYGVGTPMG SPYRDKITIA ILQLQEEGKL HMMKEKWWRG NGCPEEESKE ASALGVQNIG GIFIVLAAGL VLSVFVAVGE FLYKSKKNAQ LEKRSFCSAM VEELRMSLKC QRRLKHKPQA PVIVKTEEVI NMHTFNDRRL PGKETMA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human GRIK2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: GRIK2 (Glutamate Receptor, Ionotropic, Kainate 2 (GRIK2))

Autre désignation: GRIK2 (GRIK2 Produits)

Synonymes: GRIK5 Protein, eaa4 Protein, glr6 Protein, gluk6 Protein, glur6 Protein, grik2 Protein, mrt6 Protein, GluR6 Protein, grik2-A Protein, EAA4 Protein, GLR6 Protein, GLUK6 Protein, GLUR6 Protein, GluK2 Protein, MRT6 Protein, AW124492 Protein, Glur-6 Protein, Glur6 Protein, Glurbeta2 Protein, GRIK2 Protein, glutamate ionotropic receptor kainate type subunit 2 Protein, glutamate receptor, ionotropic, kainate 2 L homeolog Protein, glutamate receptor, ionotropic, kainate 2 Protein, glutamate receptor, ionotropic, kainate 2 (beta 2) Protein, GRIK2 Protein, grik2.L Protein, grik2 Protein, Grik2 Protein

Sujet: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Modulates cell surface expression of NETO2 (By similarity). {ECO:0000250}.

Poids moléculaire: 100.2 kDa Including tag.

UniProt: Q13002

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity