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HTRA3 Protein (AA 18-453) (His tag)

Crystallography grade HTRA3 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3092994
  • Antigène Voir toutes HTRA3 Protéines
    HTRA3 (HtrA Serine Peptidase 3 (HTRA3))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 18-453
    Origine
    • 2
    • 1
    Humain
    Source
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette HTRA3 protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    MREPPAAPCP ARCDVSRCPS PRCPGGYVPD LCNCCLVCAA SEGEPCGGPL DSPCGESLEC VRGLCRCRWS HAVCGTDGHT YANVCALQAA SRRALQLSGT PVRQLQKGAC PLGLHQLSSP RYKFNFIADV VEKIAPAVVH IELFLRHPLF GRNVPLSSGS GFIMSEAGLI ITNAHVVSSN SAAPGRQQLK VQLQNGDSYE ATIKDIDKKS DIATIKIHPK KKLPVLLLGH SADLRPGEFV VAIGSPFALQ NTVTTGIVST AQREGRELGL RDSDMDYIQT DAIINYGNSG GPLVNLDGEV IGINTLKVTA GISFAIPSDR ITRFLTEFQD KQIKDWKKRF IGIRMRTITP SLVDELKASN PDFPEVSSGI YVQEVAPNSP SQRGGIQDGD IIVKVNGRPL VDSSELQEAV LTESPLLLEV RRGNDDLLFS IAPEVVMHHHHHH
    Sequence including C-terminal His-tag.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human HTRA3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).
    • Tag Location: C-terminal His Tag

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    The protein is purified from the cleared cell lysate using EDTA resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    HTRA3 (HtrA Serine Peptidase 3 (HTRA3))
    Autre désignation
    HTRA3 (HTRA3 Produits)
    Synonymes
    HTRA3 Protein, Prsp Protein, Tasp Protein, 2210021K23Rik Protein, 9530081K03Rik Protein, RGD1308120 Protein, Trypsin-like serine protease Protein, HtrA serine peptidase 3 Protein, htrA3 Protein, HTRA3 Protein, Htra3 Protein
    Sujet
    Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (By similarity). May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (PubMed:21321049, PubMed:22229724). {ECO:0000250|UniProtKB:Q9D236, ECO:0000269|PubMed:21321049, ECO:0000269|PubMed:22229724}.
    Poids moléculaire
    47.9 kDa Including tag.
    UniProt
    P83110
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