HTRA3 Protein (AA 18-453) (His tag)
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- Antigène Voir toutes HTRA3 Protéines
- HTRA3 (HtrA Serine Peptidase 3 (HTRA3))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 18-453
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette HTRA3 protéine est marqué à la His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Séquence
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MREPPAAPCP ARCDVSRCPS PRCPGGYVPD LCNCCLVCAA SEGEPCGGPL DSPCGESLEC VRGLCRCRWS HAVCGTDGHT YANVCALQAA SRRALQLSGT PVRQLQKGAC PLGLHQLSSP RYKFNFIADV VEKIAPAVVH IELFLRHPLF GRNVPLSSGS GFIMSEAGLI ITNAHVVSSN SAAPGRQQLK VQLQNGDSYE ATIKDIDKKS DIATIKIHPK KKLPVLLLGH SADLRPGEFV VAIGSPFALQ NTVTTGIVST AQREGRELGL RDSDMDYIQT DAIINYGNSG GPLVNLDGEV IGINTLKVTA GISFAIPSDR ITRFLTEFQD KQIKDWKKRF IGIRMRTITP SLVDELKASN PDFPEVSSGI YVQEVAPNSP SQRGGIQDGD IIVKVNGRPL VDSSELQEAV LTESPLLLEV RRGNDDLLFS IAPEVVMHHHHHH
Sequence including C-terminal His-tag. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human HTRA3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
- Tag Location: C-terminal His Tag
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
- The protein is purified from the cleared cell lysate using EDTA resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Classe de qualité
- Crystallography grade
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- HTRA3 (HtrA Serine Peptidase 3 (HTRA3))
- Autre désignation
- HTRA3 (HTRA3 Produits)
- Synonymes
- HTRA3 Protein, Prsp Protein, Tasp Protein, 2210021K23Rik Protein, 9530081K03Rik Protein, RGD1308120 Protein, Trypsin-like serine protease Protein, HtrA serine peptidase 3 Protein, htrA3 Protein, HTRA3 Protein, Htra3 Protein
- Sujet
- Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (By similarity). May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (PubMed:21321049, PubMed:22229724). {ECO:0000250|UniProtKB:Q9D236, ECO:0000269|PubMed:21321049, ECO:0000269|PubMed:22229724}.
- Poids moléculaire
- 47.9 kDa Including tag.
- UniProt
- P83110
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