CD11b Protein (AA 17-1152) (rho-1D4 tag)

N° du produit ABIN3093166

Détail du produit

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Type de proteíne: Recombinant

Attributs du protein: AA 17-1152

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette CD11b protéine est marqué à la rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: FNLDTENAMT FQENARGFGQ SVVQLQGSRV VVGAPQEIVA ANQRGSLYQC DYSTGSCEPI RLQVPVEAVN MSLGLSLAAT TSPPQLLACG PTVHQTCSEN TYVKGLCFLF GSNLRQQPQK FPEALRGCPQ EDSDIAFLID GSGSIIPHDF RRMKEFVSTV MEQLKKSKTL FSLMQYSEEF RIHFTFKEFQ NNPNPRSLVK PITQLLGRTH TATGIRKVVR ELFNITNGAR KNAFKILVVI TDGEKFGDPL GYEDVIPEAD REGVIRYVIG VGDAFRSEKS RQELNTIASK PPRDHVFQVN NFEALKTIQN QLREKIFAIE GTQTGSSSSF EHEMSQEGFS AAITSNGPLL STVGSYDWAG GVFLYTSKEK STFINMTRVD SDMNDAYLGY AAAIILRNRV QSLVLGAPRY QHIGLVAMFR QNTGMWESNA NVKGTQIGAY FGASLCSVDV DSNGSTDLVL IGAPHYYEQT RGGQVSVCPL PRGRARWQCD AVLYGEQGQP WGRFGAALTV LGDVNGDKLT DVAIGAPGEE DNRGAVYLFH GTSGSGISPS HSQRIAGSKL SPRLQYFGQS LSGGQDLTMD GLVDLTVGAQ GHVLLLRSQP VLRVKAIMEF NPREVARNVF ECNDQVVKGK EAGEVRVCLH VQKSTRDRLR EGQIQSVVTY DLALDSGRPH SRAVFNETKN STRRQTQVLG LTQTCETLKL QLPNCIEDPV SPIVLRLNFS LVGTPLSAFG NLRPVLAEDA QRLFTALFPF EKNCGNDNIC QDDLSITFSF MSLDCLVVGG PREFNVTVTV RNDGEDSYRT QVTFFFPLDL SYRKVSTLQN QRSQRSWRLA CESASSTEVS GALKSTSCSI NHPIFPENSE VTFNITFDVD SKASLGNKLL LKANVTSENN MPRTNKTEFQ LELPVKYAVY MVVTSHGVST KYLNFTASEN TSRVMQHQYQ VSNLGQRSLP ISLVFLVPVR LNQTVIWDRP QVTFSENLSS TCHTKERLPS HSDFLAELRK APVVNCSIAV CQRIQCDIPF FGIQEEFNAT LKGNLSFDWY IKTSHNHLLI VSTAEILFND SVFTLLPGQG AFVRSQTETK VEPFEVPNPL PLIVGSSVGG LLLLALITAA LYKLGFFKRQ YKDMMSEGGP PGAEPQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ITGAM Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Autre désignation: ITGAM (ITGAM Produits)

Synonymes: CD11b/CD18 Protein, CR3 Protein, CR3A Protein, Cd11b Protein, F730045J24Rik Protein, Ly-40 Protein, MAC1 Protein, Mac-1 Protein, Mac-1a Protein, CD11B Protein, MAC-1 Protein, MAC1A Protein, MO1A Protein, SLEB6 Protein, C3bi Protein, integrin subunit alpha M Protein, integrin alpha M Protein, integrin, alpha M (complement component 3 receptor 3 subunit) Protein, integrin alpha-M Protein, ITGAM Protein, Itgam Protein, LOC100351865 Protein

Sujet: Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

Poids moléculaire: 126.6 kDa Including tag.

UniProt: P11215

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation