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CAMK2D Protein (AA 2-499) (His tag)

Crystallography grade CAMK2D Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
N° du produit ABIN3093227
  • Antigène Voir toutes CAMK2D Protéines
    CAMK2D (Calcium/calmodulin-Dependent Protein Kinase II delta (CAMK2D))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-499
    Origine
    • 8
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Source
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette CAMK2D protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Séquence
    ASTTTCTRFT DEYQLFEELG KGAFSVVRRC MKIPTGQEYA AKIINTKKLS ARDHQKLERE ARICRLLKHP NIVRLHDSIS EEGFHYLVFD LVTGGELFED IVAREYYSEA DASHCIQQIL ESVNHCHLNG IVHRDLKPEN LLLASKSKGA AVKLADFGLA IEVQGDQQAW FGFAGTPGYL SPEVLRKDPY GKPVDMWACG VILYILLVGY PPFWDEDQHR LYQQIKAGAY DFPSPEWDTV TPEAKDLINK MLTINPAKRI TASEALKHPW ICQRSTVASM MHRQETVDCL KKFNARRKLK GAILTTMLAT RNFSAAKSLL KKPDGVKEST ESSNTTIEDE DVKARKQEII KVTEQLIEAI NNGDFEAYTK ICDPGLTAFE PEALGNLVEG MDFHRFYFEN ALSKSNKPIH TIILNPHVHL VGDDAACIAY IRLTQYMDGS GMPKTMQSEE TRVWHRRDGK WQNVHFHRSG SPTVPIKPPC IPNGKENFSG GTSLWQNI
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human CAMK2D Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product CAMK2D Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    CAMK2D (Calcium/calmodulin-Dependent Protein Kinase II delta (CAMK2D))
    Autre désignation
    CAMK2D (CAMK2D Produits)
    Synonymes
    CAMKD Protein, CAMK1 Protein, Camk2 Protein, Camki Protein, RATCAMKI Protein, camk2 Protein, camkd Protein, camkII Protein, 2810011D23Rik Protein, 8030469K03Rik Protein, [d]-CaMKII Protein, mKIAA4163 Protein, calcium/calmodulin dependent protein kinase II delta Protein, calcium/calmodulin-dependent protein kinase II delta Protein, calcium/calmodulin dependent protein kinase (CaM kinase) II delta S homeolog Protein, calcium/calmodulin dependent protein kinase (CaM kinase) II delta Protein, calcium/calmodulin-dependent protein kinase II, delta Protein, CAMK2D Protein, Camk2d Protein, camk2d.S Protein, camk2d Protein
    Sujet
    Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca(2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca(2+) influx into the myocyte, Ca(2+) release from the sarcoplasmic reticulum (SR), SR Ca(2+) uptake and Na(+) and K(+) channel transport. Targets also transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca(2+) release via direct phosphorylation of RYR2 Ca(2+) channel on 'Ser-2808'. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program. Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca(2+) influx to myocytes by binding and phosphorylating the L-type Ca(2+) channel subunit beta-2 CACNB2. In addition to Ca(2+) channels, can target and regulate the cardiac sarcolemmal Na(+) channel Nav1.5/SCN5A and the K+ channel Kv4.3/KCND3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2, contributing to the enhancement of SR Ca(2+) uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca(2+) transport through phosphorylation of PLN/PLB and triadin, a ryanodine receptor-coupling factor. {ECO:0000269|PubMed:16690701, ECO:0000269|PubMed:17179159}.
    Poids moléculaire
    57.2 kDa Including tag.
    UniProt
    Q13557
    Pathways
    Signalisation WNT, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Smooth Muscle Cell Migration
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