Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

KIF24 Protein (AA 1-1368) (His tag)

Crystallography grade KIF24 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
N° du produit ABIN3093379
  • Antigène Tous les produits KIF24
    KIF24 (Kinesin Family Member 24 (KIF24))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-1368
    Origine
    Humain
    Source
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette KIF24 protéine est marqué à la His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Séquence
    MASWLYECLC EAELAQYYSH FTALGLQKID ELAKITMKDY SKLGVHDMND RKRLFQLIKI IKIMQEEDKA VSIPERHLQT SSLRIKSQEL RSGPRRQLNF DSPADNKDRN ASNDGFEMCS LSDFSANEQK STYLKVLEHM LPDDSQYHTK TGILNATAGD SYVQTEISTS LFSPNYLSAI LGDCDIPIIQ RISHVSGYNY GIPHSCIRQN TSEKQNPWTE MEKIRVCVRK RPLGMREVRR GEINIITVED KETLLVHEKK EAVDLTQYIL QHVFYFDEVF GEACTNQDVY MKTTHPLIQH IFNGGNATCF AYGQTGAGKT YTMIGTHENP GLYALAAKDI FRQLEVSQPR KHLFVWISFY EIYCGQLYDL LNRRKRLFAR EDSKHMVQIV GLQELQVDSV ELLLEVILKG SKERSTGATG VNADSSRSHA VIQIQIKDSA KRTFGRISFI DLAGSERAAD ARDSDRQTKM EGAEINQSLL ALKECIRALD QEHTHTPFRQ SKLTQVLKDS FIGNAKTCMI ANISPSHVAT EHTLNTLRYA DRVKELKKGI KCCTSVTSRN RTSGNSSPKR IQSSPGALSE DKCSPKKVKL GFQQSLTVAA PGSTRGKVHP LTSHPPNIPF TSAPKVSGKR GGSRGSPSQE WVIHASPVKG TVRSGHVAKK KPEESAPLCS EKNRMGNKTV LGWESRASGP GEGLVRGKLS TKCKKVQTVQ PVQKQLVSRV ELSFGNAHHR AEYSQDSQRG TPARPASEAW TNIPPHQKER EEHLRFYHQQ FQQPPLLQQK LKYQPLKRSL RQYRPPEGQL TNETPPLFHS YSENHDGAQV EELDDSDFSE DSFSHISSQR ATKQRNTLEN SEDSFFLHQT WGQGPEKQVA ERQQSLFSSP RTGDKKDLTK SWVDSRDPIN HRRAALDHSC SPSKGPVDWS RENSTSSGPS PRDSLAEKPY CSQVDFIYRQ ERGGGSSFDL RKDASQSEVS GENEGNLPSP EEDGFTISLS HVAVPGSPDQ RDTVTTPLRE VSADGPIQVT STVKNGHAVP GEDPRGQLGT HAEYASGLMS PLTMSLLENP DNEGSPPSEQ LVQDGATHSL VAESTGGPVV SHTVPSGDQE AALPVSSATR HLWLSSSPPD NKPGGDLPAL SPSPIRQHPA DKLPSREADL GEACQSRETV LFSHEHMGSE QYDADAEETG LDGSWGFPGK PFTTIHMGVP HSGPTLTPRT GSSDVADQLW AQERKHPTRL GWQEFGLSTD PIKLPCNSEN VTWLKPRPIS RCLARPSSPL VPSCSPKTAG TLRQPTLEQA QQVVIRAHQE QLDEMAELGF KEETLMSQLA SNDFEDFVTQ LDEIMVLKSK CIQSLRSQLQ LYLTCHGPTA APEGTVPS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human KIF24 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    KIF24 (Kinesin Family Member 24 (KIF24))
    Autre désignation
    KIF24 (KIF24 Produits)
    Synonymes
    C9orf48 Protein, bA571F15.4 Protein, 4933425J19Rik Protein, 9430029L23Rik Protein, RGD1307723 Protein, kinesin family member 24 Protein, KIF24 Protein, Kif24 Protein
    Sujet
    Microtubule-dependent motor protein that acts as a negative regulator of ciliogenesis by mediating recruitment of CCP110 to mother centriole in cycling cells, leading to restrict nucleation of cilia at centrioles. Mediates depolymerization of microtubules of centriolar origin, possibly to suppress aberrant cilia formation. {ECO:0000269|PubMed:21620453}.
    Poids moléculaire
    152.9 kDa Including tag.
    UniProt
    Q5T7B8
Vous êtes ici:
Support technique