LRP8 Protein (AA 42-826) (His tag)

N° du produit ABIN3093628

Détail du produit

Antigène: LRP8 (Low Density Lipoprotein Receptor-Related Protein 8, Apolipoprotein E Receptor (LRP8))

Type de proteíne: Recombinant

Attributs du protein: AA 42-826

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette LRP8 protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: GPAKDCEKDQ FQCRNERCIP SVWRCDEDDD CLDHSDEDDC PKKTCADSDF TCDNGHCIHE RWKCDGEEEC PDGSDESEAT CTKQVCPAEK LSCGPTSHKC VPASWRCDGE KDCEGGADEA GCATLCAPHE FQCGNRSCLA AVFVCDGDDD CGDGSDERGC ADPACGPREF RCGGDGGGAC IPERWVCDRQ FDCEDRSDEA AELCGRPGPG ATSAPAACAT ASQFACRSGE CVHLGWRCDG DRDCKDKSDE ADCPLGTCRG DEFQCGDGTC VLAIKHCNQE QDCPDGSDEA GCLQGLNECL HNNGGCSHIC TDLKIGFECT CPAGFQLLDQ KTCGDIDECK DPDACSQICV NYKGYFKCEC YPGYEMDLLT KNCKAAAGKS PSLIFTNRHE VRRIDLVKRN YSRLIPMLKN VVALDVEVAT NRIYWCDLSY RKIYSAYMDK ASDPKEQEVL IDEQLHSPEG LAVDWVHKHI YWTDSGNKTI SVATVDGGRR RTLFSRNLSE PRAIAVDPLR GFMYWSDWGD QAKIEKSGLN GVDRQTLVSD NIEWPNGITL DLLSQRLYWV DSKLHQLSSI DFSGGNRKTL ISSTDFLSHP FGIAVFEDKV FWTDLENEAI FSANRLNGLE ISILAENLNN PHDIVIFHEL KQPRAPDACE LSVQPNGGCE YLCLPAPQIS SHSPKYTCAC PDTMWLGPDM KRCYRAPQST STTTLASTMT RTVPATTRAP GTTVHRSTYQ NHSTETPSLT AAVPSSVSVP RAPSISPSTL SPATSNHSQH YANEDSKMGS TVTAA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human LRP8 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: LRP8 (Low Density Lipoprotein Receptor-Related Protein 8, Apolipoprotein E Receptor (LRP8))

Autre désignation: LRP8 (LRP8 Produits)

Synonymes: LRP8 Protein, APOER2 Protein, HSZ75190 Protein, LRP-8 Protein, MCI1 Protein, 4932703M08Rik Protein, AA921429 Protein, AI848122 Protein, ApoER2 Protein, Lr8b Protein, LR8B Protein, LDL receptor related protein 8 Protein, low density lipoprotein receptor-related protein 8, apolipoprotein e receptor Protein, LRP8 Protein, Lrp8 Protein

Sujet: Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. May also function as an endocytic receptor. {ECO:0000269|PubMed:12807892, ECO:0000269|PubMed:12899622, ECO:0000269|PubMed:12950167}.

Poids moléculaire: 86.9 kDa Including tag.

UniProt: Q14114