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MACC1 Protein (AA 1-852) (His tag)

Crystallography grade MACC1 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
N° du produit ABIN3093653
  • Antigène Voir toutes MACC1 Protéines
    MACC1 (Metastasis Associated in Colon Cancer 1 (MACC1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-852
    Origine
    Humain
    Source
    • 2
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette MACC1 protéine est marqué à la His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Séquence
    MLITERKHFR SGRIAQSMSE ANLIDMEAGK LSKSCNITEC QDPDLLHNWP DAFTLRGNNA SKVANPFWNQ LSASNPFLDD ITQLRNNRKR NNISILKEDP FLFCREIENG NSFDSSGDEL DVHQLLRQTS SRNSGRSKSV SELLDILDDT AHAHQSIHNS DQILLHDLEW LKNDREAYKM AWLSQRQLAR SCLDLNTISQ SPGWAQTQLA EVTIACKVNH QGGSVQLPES DITVHVPQGH VAVGEFQEVS LRAFLDPPHM LNHDLSCTVS PLLEIMLGNL NTMEALLLEM KIGAEVRKDP FSQVMTEMVC LHSLGKEGPF KVLSNCYIYK DTIQVKLIDL SQVMYLVVAA QAKALPSPAA TIWDYIHKTT SIGIYGPKYI HPSFTVVLTV CGHNYMPGQL TISDIKKGGK NISPVVFQLW GKQSFLLDKP QDLSISIFSC DPDFEVKTEG ERKEIKQKQL EAGEVVHQQF LFSLVEHREM HLFDFCVQVE PPNGEPVAQF SITTPDPTPN LKRLSNLPGY LQKKEEIKSA PLSPKILVKY PTFQDKTLNF SNYGVTLKAV LRQSKIDYFL EYFKGDTIAL LGEGKVKAIG QSKVKEWYVG VLRGKIGLVH CKNVKVISKE QVMFMSDSVF TTRNLLEQIV LPLKKLTYIY SVVLTLVSEK VYDWKVLADV LGYSHLSLED FDQIQADKES EKVSYVIKKL KEDCHTERNT RKFLYELIVA LLKMDCQELV ARLIQEAAVL TSAVKLGKGW RELAEKLVRL TKQQMEAYEI PHRGNTGDVA VEMMWKPAYD FLYTWSAHYG NNYRDVLQDL QSALDRMKNP VTKHWRELTG VLILVNSLEV LRVTAFSTSE EV
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human MACC1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    MACC1 (Metastasis Associated in Colon Cancer 1 (MACC1))
    Autre désignation
    MACC1 (MACC1 Produits)
    Synonymes
    7A5 Protein, SH3BP4L Protein, 4732474O15Rik Protein, Gm267 Protein, 7a5 Protein, RGD1309838 Protein, MACC1, MET transcriptional regulator Protein, metastasis associated in colon cancer 1 Protein, MACC1 Protein, Macc1 Protein
    Sujet
    Acts as a transcription activator for MET and as a key regulator of HGF-MET signaling. Promotes cell motility, proliferation and hepatocyte growth factor (HGF)-dependent scattering in vitro and tumor growth and metastasis in vivo. {ECO:0000269|PubMed:19098908}.
    Poids moléculaire
    97.6 kDa Including tag.
    UniProt
    Q6ZN28
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