MALT1 Protein (AA 2-824) (His tag)

N° du produit ABIN3093695

Détail du produit

Antigène: MALT1 (Mucosa Associated Lymphoid Tissue Lymphoma Translocation Gene 1 (MALT1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-824

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette MALT1 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: SLLGDPLQAL PPSAAPTGPL LAPPAGATLN RLREPLLRRL SELLDQAPEG RGWRRLAELA GSRGRLRLSC LDLEQCSLKV LEPEGSPSLC LLKLMGEKGC TVTELSDFLQ AMEHTEVLQL LSPPGIKITV NPESKAVLAG QFVKLCCRAT GHPFVQYQWF KMNKEIPNGN TSELIFNAVH VKDAGFYVCR VNNNFTFEFS QWSQLDVCDI PESFQRSVDG VSESKLQICV EPTSQKLMPG STLVLQCVAV GSPIPHYQWF KNELPLTHET KKLYMVPYVD LEHQGTYWCH VYNDRDSQDS KKVEIIIGRT DEAVECTEDE LNNLGHPDNK EQTTDQPLAK DKVALLIGNM NYREHPKLKA PLVDVYELTN LLRQLDFKVV SLLDLTEYEM RNAVDEFLLL LDKGVYGLLY YAGHGYENFG NSFMVPVDAP NPYRSENCLC VQNILKLMQE KETGLNVFLL DMCRKRNDYD DTIPILDALK VTANIVFGYA TCQGAEAFEI QHSGLANGIF MKFLKDRLLE DKKITVLLDE VAEDMGKCHL TKGKQALEIR SSLSEKRALT DPIQGTEYSA ESLVRNLQWA KAHELPESMC LKFDCGVQIQ LGFAAEFSNV MIIYTSIVYK PPEIIMCDAY VTDFPLDLDI DPKDANKGTP EETGSYLVSK DLPKHCLYTR LSSLQKLKEH LVFTVCLSYQ YSGLEDTVED KQEVNVGKPL IAKLDMHRGL GRKTCFQTCL MSNGPYQSSA ATSGGAGHYH SLQDPFHGVY HSHPGNPSNV TPADSCHCSR TPDAFISSFA HHASCHFSRS NVPVETTDEI PFSFSDRLRI SEK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human MALT1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MALT1 (Mucosa Associated Lymphoid Tissue Lymphoma Translocation Gene 1 (MALT1))

Autre désignation: MALT1 (MALT1 Produits)

Synonymes: malt1 Protein, MALT1 Protein, mlt Protein, mlt1 Protein, MLT Protein, MLT1 Protein, A630046N12 Protein, D430033E09Rik Protein, MALT paracaspase 2 Protein, MALT1 paracaspase Protein, MALT1 paracaspase S homeolog Protein, paracaspase 3 Protein, malt2 Protein, MALT1 Protein, malt1.S Protein, Malt1 Protein, PCASP3 Protein

Sujet: Enhances BCL10-induced activation of NF-kappa-B. Involved in nuclear export of BCL10. Binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity. Has ubiquitin ligase activity. MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion. {ECO:0000269|PubMed:11262391, ECO:0000269|PubMed:14695475, ECO:0000269|PubMed:18264101}.

Poids moléculaire: 93.1 kDa Including tag.

UniProt: Q9UDY8

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins