LY75/DEC-205 Protein (AA 28-1666) (His tag)

N° du produit ABIN3093704

Détail du produit

Antigène: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Type de proteíne: Recombinant

Attributs du protein: AA 28-1666

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette LY75/DEC-205 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: SGRAANDPFT IVHGNTGKCI KPVYGWIVAD DCDETEDKLW KWVSQHRLFH LHSQKCLGLD ITKSVNELRM FSCDSSAMLW WKCEHHSLYG AARYRLALKD GHGTAISNAS DVWKKGGSEE SLCDQPYHEI YTRDGNSYGR PCEFPFLIDG TWHHDCILDE DHSGPWCATT LNYEYDRKWG ICLKPENGCE DNWEKNEQFG SCYQFNTQTA LSWKEAYVSC QNQGADLLSI NSAAELTYLK EKEGIAKIFW IGLNQLYSAR GWEWSDHKPL NFLNWDPDRP SAPTIGGSSC ARMDAESGLW QSFSCEAQLP YVCRKPLNNT VELTDVWTYS DTRCDAGWLP NNGFCYLLVN ESNSWDKAHA KCKAFSSDLI SIHSLADVEV VVTKLHNEDI KEEVWIGLKN INIPTLFQWS DGTEVTLTYW DENEPNVPYN KTPNCVSYLG ELGQWKVQSC EEKLKYVCKR KGEKLNDASS DKMCPPDEGW KRHGETCYKI YEDEVPFGTN CNLTITSRFE QEYLNDLMKK YDKSLRKYFW TGLRDVDSCG EYNWATVGGR RRAVTFSNWN FLEPASPGGC VAMSTGKSVG KWEVKDCRSF KALSICKKMS GPLGPEEASP KPDDPCPEGW QSFPASLSCY KVFHAERIVR KRNWEEAERF CQALGAHLSS FSHVDEIKEF LHFLTDQFSG QHWLWIGLNK RSPDLQGSWQ WSDRTPVSTI IMPNEFQQDY DIRDCAAVKV FHRPWRRGWH FYDDREFIYL RPFACDTKLE WVCQIPKGRT PKTPDWYNPD RAGIHGPPLI IEGSEYWFVA DLHLNYEEAV LYCASNHSFL ATITSFVGLK AIKNKIANIS GDGQKWWIRI SEWPIDDHFT YSRYPWHRFP VTFGEECLYM SAKTWLIDLG KPTDCSTKLP FICEKYNVSS LEKYSPDSAA KVQCSEQWIP FQNKCFLKIK PVSLTFSQAS DTCHSYGGTL PSVLSQIEQD FITSLLPDME ATLWIGLRWT AYEKINKWTD NRELTYSNFH PLLVSGRLRI PENFFEEESR YHCALILNLQ KSPFTGTWNF TSCSERHFVS LCQKYSEVKS RQTLQNASET VKYLNNLYKI IPKTLTWHSA KRECLKSNMQ LVSITDPYQQ AFLSVQALLH NSSLWIGLFS QDDELNFGWS DGKRLHFSRW AETNGQLEDC VVLDTDGFWK TVDCNDNQPG AICYYSGNET EKEVKPVDSV KCPSPVLNTP WIPFQNCCYN FIITKNRHMA TTQDEVHTKC QKLNPKSHIL SIRDEKENNF VLEQLLYFNY MASWVMLGIT YRNKSLMWFD KTPLSYTHWR AGRPTIKNEK FLAGLSTDGF WDIQTFKVIE EAVYFHQHSI LACKIEMVDY KEEYNTTLPQ FMPYEDGIYS VIQKKVTWYE ALNMCSQSGG HLASVHNQNG QLFLEDIVKR DGFPLWVGLS SHDGSESSFE WSDGSTFDYI PWKGQTSPGN CVLLDPKGTW KHEKCNSVKD GAICYKPTKS KKLSRLTYSS RCPAAKENGS RWIQYKGHCY KSDQALHSFS EAKKLCSKHD HSATIVSIKD EDENKFVSRL MRENNNITMR VWLGLSQHSV DQSWSWLDGS EVTFVKWENK SKSGVGRCSM LIASNETWKK VECEHGFGRV VCKVPLGPD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human LY75 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Autre désignation: LY75 (LY75 Produits)

Synonymes: LY75 Protein, MGC140308 Protein, DEC205 Protein, im:7150926 Protein, si:dkey-78p20.2 Protein, LYT75 Protein, CD205 Protein, DEC-205 Protein, CLEC13B Protein, GP200-MR6 Protein, LY-75 Protein, RGD1565962 Protein, Ly-75 Protein, lymphocyte antigen 75 Protein, CD205 protein Protein, CD302 antigen Protein, LY75 Protein, Cyan7822_2917 Protein, LY5 Protein, ly75 Protein, cd205 Protein, LOC100380704 Protein, LOC100433861 Protein, LOC100483541 Protein, Ly75 Protein

Sujet: Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes. {ECO:0000250}.

Poids moléculaire: 189.8 kDa Including tag.

UniProt: O60449