MIA3 Protein (AA 23-1144) (His tag)

N° du produit ABIN3093820

Détail du produit

Antigène: MIA3 (Melanoma Inhibitory Activity Family, Member 3 (MIA3))

Type de proteíne: Recombinant

Attributs du protein: AA 23-1144

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette MIA3 protéine est marqué à la His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: QLDPSTGRRF SEHKLCADDE CSMLMYRGEA LEDFTGPDCR FVNFKKGDPV YVYYKLARGW PEVWAGSVGR TFGYFPKDLI QVVHEYTKEE LQVPTDETDF VCFDGGRDDF HNYNVEELLG FLELYNSAAT DSEKAVEKTL QDMEKNPELS KEREPEPEPV EANSEESDSV FSENTEDLQE QFTTQKHHSH ANSQANHAQG EQASFESFEE MLQDKLKVPE SENNKTSNSS QVSNEQDKID AYKLLKKEMT LDLKTKFGST ADALVSDDET TRLVTSLEDD FDEELDTEYY AVGKEDEENQ EDFDELPLLT FTDGEDMKTP AKSGVEKYPT DKEQNSNEED KVQLTVPPGI KNDDKNILTT WGDTIFSIVT GGEETRDTMD LESSSSEEEK EDDDDALVPD SKQGKPQSAT DYSDPDNVDD GLFIVDIPKT NNDKEVNAEH HIKGKGRGVQ ESKRGLVQDK TELEDENQEG MTVHSSVHSN NLNSMPAAEK GKDTLKSAYD DTENDLKGAA IHISKGMLHE EKPGEQILEG GSESESAQKA AGNQMNDRKI QQESLGSAPL MGDDHPNASR DSVEGDALVN GAKLHTLSVE HQREELKEEL VLKTQNQPRF SSPDEIDLPR ELEDEVPILG RNLPWQQERD VAATASKQMS EKIRLSEGEA KEDSLDEEFF HHKAMQGTEV GQTDQTDSTG GPAFLSKVEE DDYPSEELLE DENAINAKRS KEKNPGNQGR QFDVNLQVPD RAVLGTIHPD PEIEESKQET SMILDSEKTS ETAAKGVNTG GREPNTMVEK ERPLADKKAQ RPFERSDFSD SIKIQTPELG EVFQNKDSDY LKNDNPEEHL KTSGLAGEPE GELSKEDHEN TEKYMGTESQ GSAAAEPEDD SFHWTPHTSV EPGHSDKRED LLIISSFFKE QQSLQRFQKY FNVHELEALL QEMSSKLKSA QQESLPYNME KVLDKVFRAS ESQILSIAEK MLDTRVAENR DLGMNENNIF EEAAVLDDIQ DLIYFVRYKH STAEETATLV MAPPLEEGLG GAMEEMQPLH EDNFSREKTA ELNVQVPEEP THLDQRVIGD THASEVSQKP NTEKDLDPGP VTTEDTPMDA IDANKQPETA AEEPASVTPL EN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human MIA3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MIA3 (Melanoma Inhibitory Activity Family, Member 3 (MIA3))

Autre désignation: MIA3 (MIA3 Produits)

Synonymes: CG11098 Protein, Dmel\\CG11098 Protein, TANGO1 Protein, MIA3 Protein, 9130229H14Rik Protein, A930039G15Rik Protein, AU022327 Protein, B230399H06Rik Protein, C80126 Protein, Gm1525 Protein, Tango Protein, ARNT Protein, D320 Protein, TANGO Protein, UNQ6077 Protein, Transport and Golgi organization 1 Protein, MIA family member 3, ER export factor Protein, melanoma inhibitory activity family, member 3 Protein, melanoma inhibitory activity protein 3 Protein, melanoma inhibitory activity family member 3 Protein, melanoma inhibitory activity 3 Protein, Tango1 Protein, MIA3 Protein, LOC100055507 Protein, mia3 Protein, Mia3 Protein

Sujet: Required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. May participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. {ECO:0000269|PubMed:19269366}.

Poids moléculaire: 126.8 kDa Including tag.

UniProt: Q5JRA6