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MUC1 Protein (AA 24-1255) (rho-1D4 tag)

Crystallography grade MUC1 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N° du produit ABIN3093926
  • Antigène Voir toutes MUC1 Protéines
    MUC1 (Mucin 1 (MUC1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 24-1255
    Origine
    • 28
    • 8
    • 3
    • 3
    • 2
    Humain
    Source
    • 15
    • 13
    • 8
    • 2
    • 1
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette MUC1 protéine est marqué à la rho-1D4 tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Séquence
    SGHASSTPGG EKETSATQRS SVPSSTEKNA VSMTSSVLSS HSPGSGSSTT QGQDVTLAPA TEPASGSAAT WGQDVTSVPV TRPALGSTTP PAHDVTSAPD NKPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD TRPAPGSTAP PAHGVTSAPD NRPALGSTAP PVHNVTSASG SASGSASTLV HNGTSARATT TPASKSTPFS IPSHHSDTPT TLASHSTKTD ASSTHHSSVP PLTSSNHSTS PQLSTGVSFF FLSFHISNLQ FNSSLEDPST DYYQELQRDI SEMFLQIYKQ GGFLGLSNIK FRPGSVVVQL TLAFREGTIN VHDVETQFNQ YKTEAASRYN LTISDVSVSD VPFPFSAQSG AGVPGWGIAL LVLVCVLVAL AIVYLIALAV CQCRRKNYGQ LDIFPARDTY HPMSEYPTYH THGRYVPPSS TDRSPYEKVS AGNGGSSLSY TNPAVAATSA NL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human MUC1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin-free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product MUC1 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    MUC1 (Mucin 1 (MUC1))
    Autre désignation
    MUC1 (MUC1 Produits)
    Synonymes
    CA 15-3 Protein, CD227 Protein, EMA Protein, H23AG Protein, KL-6 Protein, MAM6 Protein, MCKD1 Protein, MUC-1 Protein, MUC-1/SEC Protein, MUC-1/X Protein, MUC1/ZD Protein, PEM Protein, PEMT Protein, PUM Protein, Muc-1 Protein, mucin 1, cell surface associated Protein, mucin 1, transmembrane Protein, MUC1 Protein, Muc1 Protein
    Sujet
    The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack., The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
    Poids moléculaire
    120.8 kDa Including tag.
    UniProt
    P15941
    Pathways
    Negative Regulation of intrinsic apoptotic Signaling
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