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NEURL Protein (AA 2-574) (His tag)

Crystallography grade NEURL Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
N° du produit ABIN3094125
  • Antigène Voir toutes NEURL Protéines
    NEURL (Neuralized Homolog (Drosophila) (NEURL))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-574
    Origine
    Humain
    Source
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette NEURL protéine est marqué à la His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Séquence
    GNNFSSIPSL PRGNPSRAPR GHPQNLKDSI GGPFPVTSHR CHHKQKHCPA VLPSGGLPAT PLLFHPHTKG SQILMDLSHK AVKRQASFCN AITFSNRPVL IYEQVRLKIT KKQCCWSGAL RLGFTSKDPS RIHPDSLPKY ACPDLVSQSG FWAKALPEEF ANEGNIIAFW VDKKGRVFHR INDSAVMLFF SGVRTADPLW ALVDVYGLTR GVQLLDSELV LPDCLRPRSF TALRRPSLRR EADDARLSVS LCDLNVPGAD GDEAAPAAGC PIPQNSLNSQ HSRALPAQLD GDLRFHALRA GAHVRILDEQ TVARVEHGRD ERALVFTSRP VRVAETIFVK VTRSGGARPG ALSFGVTTCD PGTLRPADLP FSPEALVDRK EFWAVCRVPG PLHSGDILGL VVNADGELHL SHNGAAAGMQ LCVDASQPLW MLFGLHGTIT QIRILGSTIL AERGIPSLPC SPASTPTSPS ALGSRLSDPL LSTCSSGPLG SSAGGTAPNS PVSLPESPVT PGLGQWSDEC TICYEHAVDT VIYTCGHMCL CYACGLRLKK ALHACCPICR RPIKDIIKTY RSS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human NEURL1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    NEURL (Neuralized Homolog (Drosophila) (NEURL))
    Autre désignation
    NEURL1 (NEURL Produits)
    Synonymes
    Xneur Protein, neurl-A Protein, zgc:153971 Protein, si:dkey-82d4.1 Protein, si:dkey-82d4.3 Protein, h-neu Protein, rnf67 Protein, neurl1 Protein, neuralized Protein, NEURL1 Protein, RNF67 Protein, bA416N2.1 Protein, neu Protein, neu-1 Protein, 2410129E16Rik Protein, AI450910 Protein, AI481072 Protein, Neu1 Protein, Neur1 Protein, Neurl Protein, Nlz Protein, Rnf67 Protein, Neurl1a Protein, NEURL Protein, neurlb Protein, zgc:153175 Protein, neuralized E3 ubiquitin protein ligase 1 L homeolog Protein, neuralized E3 ubiquitin protein ligase 1 Protein, neuralized E3 ubiquitin protein ligase 1Aa Protein, neuralized E3 ubiquitin protein ligase 1A Protein, neuralized E3 ubiquitin protein ligase 1Ab Protein, neurl1.L Protein, NEURL1 Protein, neurl1aa Protein, neurl1 Protein, Neurl1a Protein, Neurl1 Protein, neurl1ab Protein
    Sujet
    Plays a role in hippocampal-dependent synaptic plasticity, learning and memory. Involved in the formation of spines and functional synaptic contacts by modulating the translational activity of the cytoplasmic polyadenylation element-binding protein CPEB3. Promotes ubiquitination of CPEB3, and hence induces CPEB3-dependent mRNA translation activation of glutamate receptor GRIA1 and GRIA2. Can function as an E3 ubiquitin-protein ligase to activate monoubiquitination of JAG1 (in vitro), thereby regulating the Notch pathway. Acts as a tumor suppressor, inhibits malignant cell transformation of medulloblastoma (MB) cells by inhibiting the Notch signaling pathway. {ECO:0000269|PubMed:20847082}.
    Poids moléculaire
    62.7 kDa Including tag.
    UniProt
    O76050
    Pathways
    Signalisation Notch, EGFR Signaling Pathway
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