NPHP3 Protein (AA 2-1330) (His tag)

N° du produit ABIN3094158

Détail du produit

Antigène: NPHP3 (Nephronophthisis 3 (Adolescent) (NPHP3))

Type de proteíne: Recombinant

Attributs du protein: AA 2-1330

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette NPHP3 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: GTASSLVSPA GGEVIEDTYG AGGGEACEIP VEVKPKARLL RNSFRRGAGA AAGAGPGSLP RGVGAGGLLG ASFKSTGSSV PELEYAAAEY ERLRKEYEIF RVSKNQELLS MGRREAKLDT ENKRLRAELQ ALQKTYQKIL REKESALEAK YQAMERAATF EHDRDKVKRQ FKIFRETKEN EIQDLLRAKR ELESKLQRLQ AQGIQVFDPG ESDSDDNCTD VTAAGTQCEY WTGGALGSEP SIGSMIQLQQ SFRGPEFAHS SIDVEGPFAN VNRDDWDIAV ASLLQVTPLF SHSLWSNTVR CYLIYTDETQ PEMDLFLKDY SPKLKRMCET MGYFFHAVYF PIDVENQYLT VRKWEIEKSS LVILFIHLTL PSLLLEDCEE AFLKNPEGKP RLIFHRLEDG KVSSDSVQQL IDQVSNLNKT SKAKIIDHSG DPAEGVYKTY ICVEKIIKQD ILGFENTDLE TKDLGSEDSI PEEDDFGDVL WDIHDEQEQM ETFQQASNSA HELGFEKYYQ RLNDLVAAPA PIPPLLVSGG PGSGKSLLLS KWIQLQQKNS PNTLILSHFV GRPMSTSSES SLIIKRLTLK LMQHSWSVSA LTLDPAKLLE EFPRWLEKLS ARHQGSIIIV IDSIDQVQQV EKHMKWLIDP LPVNVRVIVS VNVETCPPAW RLWPTLHLDP LSPKDAKSII IAECHSVDIK LSKEQEKKLE RHCRSATTCN ALYVTLFGKM IARAGRAGNL DKILHQCFQC QDTLSLYRLV LHSIRESMAN DVDKELMKQI LCLVNVSHNG VSESELMELY PEMSWTFLTS LIHSLYKMCL LTYGCGLLRF QHLQAWETVR LEYLEGPTVT SSYRQKLINY FTLQLSQDRV TWRSADELPW LFQQQGSKQK LHDCLLNLFV SQNLYKRGHF AELLSYWQFV GKDKSAMATE YFDSLKQYEK NCEGEDNMSC LADLYETLGR FLKDLGLLSQ AIVPLQRSLE IRETALDPDH PRVAQSLHQL ASVYVQWKKF GNAEQLYKQA LEISENAYGA DHPYTARELE ALATLYQKQN KYEQAEHFRK KSFKIHQKAI KKKGNLYGFA LLRRRALQLE ELTLGKDTPD NARTLNELGV LYYLQNNLET ADQFLKRSLE MRERVLGPDH PDCAQSLNNL AALCNEKKQY DKAEELYERA LDIRRRALAP DHPSLAYTVK HLAILYKKMG KLDKAVPLYE LAVEIRQKSF GPKHPSVATA LVNLAVLYSQ MKKHVEALPL YERALKIYED SLGRMHPRVG ETLKNLAVLS YEGGDFEKAA ELYKRAMEIK EAETSLLGGK APSRHSSSGD TFSLKTAHSP NVFLQQGQR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human NPHP3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: NPHP3 (Nephronophthisis 3 (Adolescent) (NPHP3))

Autre désignation: NPHP3 (NPHP3 Produits)

Synonymes: si:dkey-56l10.1 Protein, MKS7 Protein, NPH3 Protein, RHPD Protein, 3632410F03Rik Protein, AI550417 Protein, C230078J01 Protein, D330020E01Rik Protein, pcy Protein, RGD1563856 Protein, nephronophthisis 3 Protein, nephrocystin 3 Protein, nephronophthisis 3 (adolescent) Protein, nphp3 Protein, NPHP3 Protein, Nphp3 Protein

Sujet: Required for normal ciliary development and function. Inhibits disheveled-1-induced canonical Wnt-signaling activity and may also play a role in the control of non-canonical Wnt signaling which regulates planar cell polarity. Probably acts as a molecular switch between different Wnt signaling pathways. Required for proper convergent extension cell movements. {ECO:0000269|PubMed:18371931}.

Poids moléculaire: 151.7 kDa Including tag.

UniProt: Q7Z494

Pathways: cAMP Metabolic Process