Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

NUP153 Protein (AA 2-1475) (His tag)

Crystallography grade NUP153 Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
N° du produit ABIN3094271
  • Antigène Voir toutes NUP153 Protéines
    NUP153 (Nucleoporin 153kDa (NUP153))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-1475
    Origine
    Humain
    Source
    • 2
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette NUP153 protéine est marqué à la His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Séquence
    ASGAGGVGGG GGGKIRTRRC HQGPIKPYQQ GRQQHQGILS RVTESVKNIV PGWLQRYFNK NEDVCSCSTD TSEVPRWPEN KEDHLVYADE ESSNITDGRI TPEPAVSNTE EPSTTSTASN YPDVLTRPSL HRSHLNFSML ESPALHCQPS TSSAFPIGSS GFSLVKEIKD STSQHDDDNI STTSGFSSRA SDKDITVSKN TSLPPLWSPE AERSHSLSQH TATSSKKPAF NLSAFGTLSP SLGNSSILKT SQLGDSPFYP GKTTYGGAAA AVRQSKLRNT PYQAPVRRQM KAKQLSAQSY GVTSSTARRI LQSLEKMSSP LADAKRIPSI VSSPLNSPLD RSGIDITDFQ AKREKVDSQY PPVQRLMTPK PVSIATNRSV YFKPSLTPSG EFRKTNQRID NKCSTGYEKN MTPGQNREQR ESGFSYPNFS LPAANGLSSG VGGGGGKMRR ERTRFVASKP LEEEEMEVPV LPKISLPITS SSLPTFNFSS PEITTSSPSP INSSQALTNK VQMTSPSSTG SPMFKFSSPI VKSTEANVLP PSSIGFTFSV PVAKTAELSG SSSTLEPIIS SSAHHVTTVN STNCKKTPPE DCEGPFRPAE ILKEGSVLDI LKSPGFASPK IDSVAAQPTA TSPVVYTRPA ISSFSSSGIG FGESLKAGSS WQCDTCLLQN KVTDNKCIAC QAAKLSPRDT AKQTGIETPN KSGKTTLSAS GTGFGDKFKP VIGTWDCDTC LVQNKPEAIK CVACETPKPG TCVKRALTLT VVSESAETMT ASSSSCTVTT GTLGFGDKFK RPIGSWECSV CCVSNNAEDN KCVSCMSEKP GSSVPASSSS TVPVSLPSGG SLGLEKFKKP EGSWDCELCL VQNKADSTKC LACESAKPGT KSGFKGFDTS SSSSNSAASS SFKFGVSSSS SGPSQTLTST GNFKFGDQGG FKIGVSSDSG SINPMSEGFK FSKPIGDFKF GVSSESKPEE VKKDSKNDNF KFGLSSGLSN PVSLTPFQFG VSNLGQEEKK EELPKSSSAG FSFGTGVINS TPAPANTIVT SENKSSFNLG TIETKSASVA PFTCKTSEAK KEEMPATKGG FSFGNVEPAS LPSASVFVLG RTEEKQQEPV TSTSLVFGKK ADNEEPKCQP VFSFGNSEQT KDENSSKSTF SFSMTKPSEK ESEQPAKATF AFGAQTSTTA DQGAAKPVFS FLNNSSSSSS TPATSAGGGI FGSSTSSSNP PVATFVFGQS SNPVSSSAFG NTAESSTSQS LLFSQDSKLA TTSSTGTAVT PFVFGPGASS NNTTTSGFGF GATTTSSSAG SSFVFGTGPS APSASPAFGA NQTPTFGQSQ GASQPNPPGF GSISSSTALF PTGSQPAPPT FGTVSSSSQP PVFGQQPSQS AFGSGTTPNS SSAFQFGSST TNFNFTNNSP SGVFTFGANS STPAASAQPS GSGGFPFNQS PAAFTVGSNG KNVFSSSGTS FSGRKIKTAV RRRK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human NUP153 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product NUP153 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    NUP153 (Nucleoporin 153kDa (NUP153))
    Autre désignation
    NUP153 (NUP153 Produits)
    Synonymes
    CG4453 Protein, Dmel\\CG4453 Protein, NUP153 Protein, Nup 153 Protein, dNup153 Protein, dmNup153 Protein, nup153 Protein, NUCZINK Protein, B130015D15Rik Protein, C88147 Protein, HNUP153 Protein, N153 Protein, Nucleoporin 153kD Protein, nucleoporin 153 Protein, nucleoporin 153kDa L homeolog Protein, Nup153 Protein, NUP153 Protein, nup153.L Protein
    Sujet
    Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs. Involved in the quality control and retention of unspliced mRNAs in the nucleus, in association with TPR, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Mediates TPR anchoring to the nuclear membrane at NPC. The repeat-containing domain may be involved in anchoring other components of the NPC to the pore membrane. Possible DNA-binding subunit of the nuclear pore complex (NPC). {ECO:0000269|PubMed:12802065, ECO:0000269|PubMed:15229283, ECO:0000269|PubMed:22253824}.
    Poids moléculaire
    154.8 kDa Including tag.
    UniProt
    P49790
    Pathways
    Protein targeting to Nucleus
Vous êtes ici:
Support technique