PTGS2 Protein (AA 18-604) (His tag)

N° du produit ABIN3094518

Détail du produit

Antigène: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Type de proteíne: Recombinant

Attributs du protein: AA 18-604

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette PTGS2 protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: ANPCCSHPCQ NRGVCMSVGF DQYKCDCTRT GFYGENCSTP EFLTRIKLFL KPTPNTVHYI LTHFKGFWNV VNNIPFLRNA IMSYVLTSRS HLIDSPPTYN ADYGYKSWEA FSNLSYYTRA LPPVPDDCPT PLGVKGKKQL PDSNEIVEKL LLRRKFIPDP QGSNMMFAFF AQHFTHQFFK TDHKRGPAFT NGLGHGVDLN HIYGETLARQ RKLRLFKDGK MKYQIIDGEM YPPTVKDTQA EMIYPPQVPE HLRFAVGQEV FGLVPGLMMY ATIWLREHNR VCDVLKQEHP EWGDEQLFQT SRLILIGETI KIVIEDYVQH LSGYHFKLKF DPELLFNKQF QYQNRIAAEF NTLYHWHPLL PDTFQIHDQK YNYQQFIYNN SILLEHGITQ FVESFTRQIA GRVAGGRNVP PAVQKVSQAS IDQSRQMKYQ SFNEYRKRFM LKPYESFEEL TGEKEMSAEL EALYGDIDAV ELYPALLVEK PRPDAIFGET MVEVGAPFSL KGLMGNVICS PAYWKPSTFG GEVGFQIINT ASIQSLICNN VKGCPFTSFS VPDPELIKTV TINASSSRSG LDDINPTVLL KERSTEL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PTGS2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Autre désignation: PTGS2 (PTGS2 Produits)

Synonymes: COX-2 Protein, COX2 Protein, GRIPGHS Protein, PGG/HS Protein, PGHS-2 Protein, PHS-2 Protein, hCox-2 Protein, Cox-2 Protein, Pghs2 Protein, TIS10 Protein, Cox2 Protein, cox-2 Protein, CEF147 Protein, PGHS2 Protein, PHSII Protein, cox2 Protein, gripghs Protein, pgg/hs Protein, pghs-2 Protein, phs-2 Protein, PTGS2 Protein, hcox-2 Protein, fj02a10 Protein, ptgs2 Protein, unp1239 Protein, wu:fj02a10 Protein, zCOX-2 Protein, si:dkey-97o5.6 Protein, prostaglandin-endoperoxide synthase 2 Protein, prostaglandin G/H synthase 2 Protein, prostaglandin-endoperoxide synthase 2S homeolog Protein, prostaglandin-endoperoxide synthase 2a Protein, prostaglandin-endoperoxide synthase 2b Protein, PTGS2 Protein, Ptgs2 Protein, LOC100136456 Protein, ptgs2.S Protein, ptgs2 Protein, ptgs2a Protein, ptgs2b Protein

Sujet: Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. {ECO:0000269|PubMed:16373578}.

Poids moléculaire: 68.2 kDa Including tag.

UniProt: P35354

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation