ERVK-9 Protein (AA 2-1117) (His tag)

N° du produit ABIN3094735

Détail du produit

Antigène: ERVK-9 (Endogenous Retrovirus Group K, Member 9 (ERVK-9))

Type de proteíne: Recombinant

Attributs du protein: AA 2-1117

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette ERVK-9 protéine est marqué à la His tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Séquence: GQTKSKIKSK YASYLSFIKI LLKRGGVKVS TKNLIKLFQI IEQFCPWFPE QGTLDLKDWK RIGKELKQAG RKGNIIPLTV WNDWAIIKAA LEPFQTEEDS ISVSDAPGSG IIDCNEKTRK KSQKETESLH CEYVAEPVMA QSTQNVDYNQ LQEVIYPETL KLEGKGPELV GPSESKPRGT SPLPAGQVPV TLQPQKQVKE NKTQPPVAYQ YWPPAELQYR PPPESQYGYP GMPPAPQGRA PYPQPPTRRL NPTAPPSRQG SELHEIIDKS RKEGDTEAWQ FPVTLEPMPP GEGAQEGEPP TVEARYKSFS IKILKDMKEG VKQYGPNSPY MRTLLDSIAH GHRLIPYDWE ILAKSSLSPS QFLQFKTWWI DGVQEQVRRN RAANPPVNID ADQLLGIGQN WSTISQQALM QNEAIEQVRA ICLRAWEKIQ DPGSTCPSFN TVRQGSKEPY PDFVARLQDV AQKSIADEKA RKVIVELMAY ENANPECQSA IKPLKGKVPA GSDVISEYVK ACDGIGGAMH KAMLMAQAIT GVVLGGQVRT FGGKCYNCGQ IGHLKKNCPV LNKQNITIQA TTTGREPPDL CPRCKKGKHW ASQCRSKFDK NGQPLSGNEQ RGQPQAPQQT GAFPIQPFVP QGFQGQQPPL SQVFQGISQL PQYNNCPPPQ VAVQQVDLCT IQAVSLLPGE PPQKIPTGVY GPLPEGTVGL ILGRSSLNLK GVQIHTSVVD SDYKGEIQLV ISSSVPWSAS PGDRIAQLLL LPYIKGGNSE IKRIGGLGST DPTGKAAYWA SQVSENRPVC KAIIQGKQFE GLVDTGADVS IIALNQWPKN WPKQKAVTGL VGIGTASEVY QSMEILHCLG PDNQESTVQP MITSIPLNLW GRDLLQQWGA EITMPAPLYS PTSQKIMTKR GYIPGKGLGK NEDGIKIPFE AKINQKREGI GYPFLGAATI EPPKPIPLTW KTEKPVWVNQ WPLPKQKLEA LHLLANEQLE KGHIEPSFSP WNSPVFVIQK KSGKWRMLTD LRAVNAVIQP MGPLQPGLPS PAMIPKDWPL IIIDLKDCFF TIPLAEQDCE KFAFTIPAIN NKEPATRFQW KVLPQGMLNS PTICQTFVGR ALQPVKVFRL LYYSLY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ERVK-9 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ERVK-9 (Endogenous Retrovirus Group K, Member 9 (ERVK-9))

Autre désignation: ERVK-9 (ERVK-9 Produits)

Synonymes: PR Protein, Protease Protein, Proteinase Protein, polymerase Protein, endogenous retrovirus group K member 9 Protein, ERVK-9 Protein

Sujet: The products of the Gag polyproteins of infectious retroviruses perform highly complex orchestrated tasks during the assembly, budding, maturation, and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Endogenous Gag proteins may have kept, lost or modified their original function during evolution (By similarity). {ECO:0000250}., Early post-infection, the reverse transcriptase converts the viral RNA genome into double-stranded viral DNA. The RNase H domain of the reverse transcriptase performs two functions. It degrades the RNA template and specifically removes the RNA primer from the RNA/DNA hybrid. Following nuclear import, the integrase catalyzes the insertion of the linear, double-stranded viral DNA into the host cell chromosome. Endogenous Pol proteins may have kept, lost or modified their original function during evolution (By similarity). {ECO:0000250}.

Poids moléculaire: 124.4 kDa Including tag.

UniProt: P63128