Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

PTPRJ Protein (AA 997-1337) (His tag)

Crystallography grade PTPRJ Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N° du produit ABIN3094860
  • Antigène Voir toutes PTPRJ Protéines
    PTPRJ (Protein tyrosine Phosphatase, Receptor Type, J (PTPRJ))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 997-1337
    Origine
    • 6
    • 4
    • 1
    Humain
    Source
    • 5
    • 4
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette PTPRJ protéine est marqué à la His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Séquence
    RKKRKDAKNN EVSFSQIKPK KSKLIRVENF EAYFKKQQAD SNCGFAEEYE DLKLVGISQP KYAAELAENR GKNRYNNVLP YDISRVKLSV QTHSTDDYIN ANYMPGYHSK KDFIATQGPL PNTLKDFWRM VWEKNVYAII MLTKCVEQGR TKCEEYWPSK QAQDYGDITV AMTSEIVLPE WTIRDFTVKN IQTSESHPLR QFHFTSWPDH GVPDTTDLLI NFRYLVRDYM KQSPPESPIL VHCSAGVGRT GTFIAIDRLI YQIENENTVD VYGIVYDLRM HRPLMVQTED QYVFLNQCVL DIVRSQKDSK VDLIYQNTTA MTIYENLAPV TTFGKTNGYI A
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PTPRJ Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product PTPRJ Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    PTPRJ (Protein tyrosine Phosphatase, Receptor Type, J (PTPRJ))
    Autre désignation
    PTPRJ (PTPRJ Produits)
    Synonymes
    HAAP Protein, CD148 Protein, DEP1 Protein, HPTPeta Protein, R-PTP-ETA Protein, SCC1 Protein, AI450271 Protein, BET Protein, Byp Protein, DEP-1 Protein, PTPbeta2 Protein, Ptpb2 Protein, RPTPJ Protein, Scc-1 Protein, Scc1 Protein, PTPRJ Protein, acid phosphatase 1 Protein, protein tyrosine phosphatase, receptor type J Protein, protein tyrosine phosphatase, receptor type, J Protein, ACP1 Protein, PTPRJ Protein, Ptprj Protein, EUBREC_2762 Protein
    Sujet
    Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration, through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation, through KDR dephosphorylation. Negative regulator of EGFR signaling pathway, through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling. {ECO:0000269|PubMed:10821867, ECO:0000269|PubMed:11259588, ECO:0000269|PubMed:12062403, ECO:0000269|PubMed:12370829, ECO:0000269|PubMed:12475979, ECO:0000269|PubMed:12913111, ECO:0000269|PubMed:14709717, ECO:0000269|PubMed:16682945, ECO:0000269|PubMed:16778204, ECO:0000269|PubMed:18348712, ECO:0000269|PubMed:18936167, ECO:0000269|PubMed:19332538, ECO:0000269|PubMed:19494114, ECO:0000269|PubMed:19836242, ECO:0000269|PubMed:19922411, ECO:0000269|PubMed:21091576, ECO:0000269|PubMed:21262971, ECO:0000269|PubMed:9531590, ECO:0000269|PubMed:9780142}.
    Poids moléculaire
    40.5 kDa Including tag.
    UniProt
    Q12913
    Pathways
    EGFR Signaling Pathway, Platelet-derived growth Factor Receptor Signaling
Vous êtes ici:
Support technique