RBX1 Protein (AA 2-108) (His tag)

N° du produit ABIN3094902

Détail du produit

Antigène: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-108

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette RBX1 protéine est marqué à la His tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)

Séquence: AAAMDVDTPS GTNSGAGKKR FEVKKWNAVA LWAWDIVVDN CAICRNHIMD LCIECQANQA SATSEECTVA WGVCNHAFHF HCISRWLKTR QVCPLDNREW EFQKYGH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human RBX1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Autre désignation: RBX1 (RBX1 Produits)

Synonymes: BA554C12.1 Protein, RNF75 Protein, ROC1 Protein, 1500002P15Rik Protein, AA517855 Protein, im:7137515 Protein, zgc:136444 Protein, RBX1 Protein, ATRBX1 Protein, F7C8.160 Protein, F7C8_160 Protein, HRT1 Protein, REGULATOR OF CULLINS-1 Protein, RING-BOX 1 Protein, RING-box 1 Protein, hop21 Protein, BEST:CK01110 Protein, CG16982 Protein, CK01110 Protein, Dmel\CG16982 Protein, EG:115C2.11 Protein, Rbx1 Protein, Rbx1/Roc Protein, Roc Protein, Roc1alpha Protein, anon-EST:Posey65 Protein, dRbx1 Protein, dRoc1 Protein, dRoc1a Protein, ring-box 1 Protein, ring-box 1, E3 ubiquitin protein ligase Protein, RING-box 1 Protein, RING-box protein 1 Protein, RING-box protein 1a Protein, Regulator of cullins 1a Protein, RBX1 Protein, Rbx1 Protein, rbx1 Protein, MGG_08844 Protein, LOC9308017 Protein, rbx-1 Protein, Roc1a Protein

Sujet: E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. {ECO:0000269|PubMed:10579999, ECO:0000269|PubMed:11027288, ECO:0000269|PubMed:15983046, ECO:0000269|PubMed:16678110, ECO:0000269|PubMed:16751180, ECO:0000269|PubMed:18397884, ECO:0000269|PubMed:19112177, ECO:0000269|PubMed:19679664}.

Poids moléculaire: 13.1 kDa Including tag.

UniProt: P62877

Pathways: Cycle Cellulaire, M Phase, SARS-CoV-2 Protein Interactome