c-Rel Protein (AA 2-619) (His tag)

N° du produit ABIN3094924

Détail du produit

Antigène: c-Rel (REL proto-oncogene (c-Rel))

Type de proteíne: Recombinant

Attributs du protein: AA 2-619

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette c-Rel protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: ASGAYNPYIE IIEQPRQRGM RFRYKCEGRS AGSIPGEHST DNNRTYPSIQ IMNYYGKGKV RITLVTKNDP YKPHPHDLVG KDCRDGYYEA EFGQERRPLF FQNLGIRCVK KKEVKEAIIT RIKAGINPFN VPEKQLNDIE DCDLNVVRLC FQVFLPDEHG NLTTALPPVV SNPIYDNRAP NTAELRICRV NKNCGSVRGG DEIFLLCDKV QKDDIEVRFV LNDWEAKGIF SQADVHRQVA IVFKTPPYCK AITEPVTVKM QLRRPSDQEV SESMDFRYLP DEKDTYGNKA KKQKTTLLFQ KLCQDHVETG FRHVDQDGLE LLTSGDPPTL ASQSAGITVN FPERPRPGLL GSIGEGRYFK KEPNLFSHDA VVREMPTGVS SQAESYYPSP GPISSGLSHH ASMAPLPSSS WSSVAHPTPR SGNTNPLSSF STRTLPSNSQ GIPPFLRIPV GNDLNASNAC IYNNADDIVG MEASSMPSAD LYGISDPNML SNCSVNMMTT SSDSMGETDN PRLLSMNLEN PSCNSVLDPR DLRQLHQMSS SSMSAGANSN TTVFVSQSDA FEGSDFSCAD NSMINESGPS NSTNPNSHGF VQDSQYSGIG SMQNEQLSDS FPYEFFQV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human REL Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: c-Rel (REL proto-oncogene (c-Rel))

Autre désignation: REL (c-Rel Produits)

Synonymes: C-Rel Protein, c-Rel Protein, NFkB Protein, c-rel Protein, zgc:100833 Protein, Xrel2 Protein, Xrel3 Protein, rel Protein, rel-A Protein, rel2 Protein, rel3 Protein, v-rel Protein, xrel Protein, REL proto-oncogene, NF-kB subunit Protein, reticuloendotheliosis oncogene Protein, RELA proto-oncogene, NF-kB subunit Protein, v-rel avian reticuloendotheliosis viral oncogene homolog Protein, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog Protein, ribonuclease A family member 2 pseudogene Protein, REL Protein, Rel Protein, Rela Protein, rel Protein, rel.L Protein, ECRP Protein

Sujet: Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator.

Poids moléculaire: 69.3 kDa Including tag.

UniProt: Q04864