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RASGRF1 Protein (AA 1-1273) (Strep Tag)

Crystallography grade RASGRF1 Origine: Humain Hôte: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
N° du produit ABIN3095012
  • Antigène Voir toutes RASGRF1 Protéines
    RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-1273
    Origine
    • 2
    • 1
    Humain
    Source
    • 1
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Purification/Conjugué
    Cette RASGRF1 protéine est marqué à la Strep Tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    Séquence
    MQKGIRLNDG HVASLGLLAR KDGTRKGYLS KRSSDNTKWQ TKWFALLQNL LFYFESDSSS RPSGLYLLEG CVCDRAPSPK PALSAKEPLE KQHYFTVNFS HENQKALELR TEDAKDCDEW VAAIAHASYR TLATEHEALM QKYLHLLQIV ETEKTVAKQL RQQIEDGEIE IERLKAEITS LLKDNERIQS TQTVAPNDED SDIKKIKKVQ SFLRGWLCRR KWKTIIQDYI RSPHADSMRK RNQVVFSMLE AEAEYVQQLH ILVNNFLRPL RMAASSKKPP ITHDDVSSIF LNSETIMFLH QIFYQGLKAR ISSWPTLVLA DLFDILLPML NIYQEFVRNH QYSLQILAHC KQNRDFDKLL KHYEAKPDCE ERTLETFLTY PMFQIPRYIL TLHELLAHTP HEHVERNSLD YAKSKLEELS RIMHDEVSET ENIRKNLAIE RMIIEGCEIL LDTSQTFVRQ GSLIQVPMSE KGKITRGRLG SLSLKKEGER QCFLFSKHLI ICTRGSGGKL HLTKNGVISL IDCTLLEEPE STEEEAKGSG QDIDHLDFKI GVEPKDSPPF TVILVASSRQ EKAAWTSDIS QCVDNIRCNG LMMNAFEENS KVTVPQMIKR TREGTREAEM SRSDASLYCD DVDIRFSKTM NSCKVLQIRY ASVERLLERL TDLRFLSIDF LNTFLHSYRV FTTAIVVLDK LITIYKKPIS AIPARWLRSL ELLFASGQNN KLLYGEPPKS PRATRKFSSP PPLSITKTSS PSRRRKLSLN IPIITGGKAL DLAALSCNSN GYTSMYSAMS PFSKATLDTS KLYVSSSFTN KIPDEGDTTP EKPEDPSALS KQSSEVSMRE ESDIDQNQSD DGDTETSPTK SPTTPKSVKN KNSSEFPLFS YNNGVVMTSC RELDNNRSAL SAASAFAIAT AGANEGTPNK EKYRRMSLAS AGFPPDQRNG DKEFVIRRAA TNRVLNVLRH WVSKHSQDFE TNDELKCKVI GFLEEVMHDP ELLTQERKAA ANIIRTLTQE DPGDNQITLE EITQMAEGVK AEPFENHSAL EIAEQLTLLD HLVFKKIPYE EFFGQGWMKL EKNERTPYIM KTTKHFNDIS NLIASEIIRN EDINARVSAI EKWVAVADIC RCLHNYNAVL EITSSMNRSA IFRLKKTWLK VSKQTKALID KLQKLVSSEG RFKNLREALK NCDPPCVPYL GMYLTDLAFI EEGTPNYTED GLVNFSKMRM ISHIIREIRQ FQQTAYKIEH QAKVTQYLLD QSFVMDEESL YESSLRIEPK LPT
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Attributs du produit
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    niveau d'endotoxine
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product RASGRF1 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))
    Autre désignation
    RASGRF1 (RASGRF1 Produits)
    Synonymes
    CDC25 Protein, CDC25L Protein, GNRP Protein, GRF1 Protein, GRF55 Protein, H-GRF55 Protein, PP13187 Protein, ras-GRF1 Protein, AI844718 Protein, CDC25Mm Protein, Grf1 Protein, Grfbeta Protein, P190-A Protein, Ras-GRF1 Protein, p190 Protein, p190RhoGEF Protein, cdc25 Protein, rasgrf1 Protein, Ras protein specific guanine nucleotide releasing factor 1 Protein, RAS protein-specific guanine nucleotide-releasing factor 1 Protein, Ras protein specific guanine nucleotide releasing factor 1 S homeolog Protein, si:ch211-234p18.3 Protein, RASGRF1 Protein, Rasgrf1 Protein, rasgrf1.S Protein, si:ch211-234p18.3 Protein, rasgrf1 Protein
    Sujet
    Ras-specific guanine nucleotide-releasing factor 1 (Ras-GRF1) (Guanine nucleotide-releasing protein) (GNRP) (Ras-specific nucleotide exchange factor CDC25),FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. {ECO:0000269|PubMed:11389730}.
    Poids moléculaire
    145.2 kDa
    UniProt
    Q13972
    Pathways
    Signalisation RTK, Cycle Cellulaire, M Phase
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